Linked Life Data

7941845

Source:http://linkedlifedata.com/resource/pubmed/id/7941845

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-11-14
pubmed:abstractText
Eight monoclonal antibodies (MAbs) were tested for the use in immunogold staining of Sindbis virus (SIN) antigen in cultured Baby Hamster kidney (BHK) cells. The antibodies were directed against the capsid (C) protein (5) or against the glycoprotein E1 (3), respectively. Four out of five anti-C MAbs and two out of three anti-E1 Mabs reacted on frozen sections, whereas two of the anti-C antibodies and none of the anti-E1 antibodies reacted on Lowicryl K4M-embedded cells. The MAbs reacting, in particular the anti-C Mabs which react on Lowicryl K4M-sections, will allow further studies of the pathogenesis and replication of alphaviruses on the cellular level. The results also confirm the conformation-dependence of the glycoprotein epitopes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0514-7166
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-41
pubmed:dateRevised
2008-2-13
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Demonstration of Sindbis virus antigen in Lowicryl-embedded cultured cells by immunogold staining.
pubmed:affiliation
Institute for Pathology, Hannover Veterinary School, F.R.G.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't