Linked Life Data

7940597

Source:http://linkedlifedata.com/resource/pubmed/id/7940597

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-10-31
pubmed:abstractText
Phenobarbital (PB), a long-acting barbiturate, is also a known tumor promoter. The mechanism responsible for the promoting effect of PB has not yet been elucidated. Changes in intracellular pH (pHin) have been associated with both early and late events of multistage carcinogenesis. We conducted this study to evaluate whether PB alters pH homeostasis. Adult rat liver (ARL) epithelial cells were cultured for 48 h on glass coverslips, loaded with the intracellular pH indicator SNARF-1, and perfused with various concentrations of PB to evaluate its effect on pHin. Our results show that PB treatment of cultured cells induces a concentration-dependent cytosolic acidification. These results indicate that its ability to decrease pHin may be involved in the mechanism of tumor promotion by PB.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0378-4274
pubmed:author
pubmed:issnType
Print
pubmed:volume
74
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
157-66
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Phenobarbital induces cytosolic acidification in an established liver epithelial cell line.
pubmed:affiliation
Department of Pharmacology, University of Arizona, College of Medicine, Arizona Health Sciences Center, Tucson 85724.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't