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pubmed-article:7939720pubmed:abstractTextTransgenic Xenopus laevis embryos were produced by transplantation of transfected cultured cell nuclei into unfertilized eggs. A Xenopus cell line, X-C, was stably transfected with plasmids containing a hygromycin-resistance gene and genes for either beta-galactosidase with a heat shock promoter or chloramphenicol acetyltransferase (CAT) with a muscle-specific actin promoter. Nuclei transplanted from these cells into unfertilized eggs directed development of embryos containing stably integrated copies of the plasmids in each cell. Transgenic embryos showed somite-specific expression of CAT and uniform expression of beta-galactosidase. Transgenic embryos produced by nuclear transplantation should be useful for testing the function of cloned genes in amphibian development.lld:pubmed
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pubmed-article:7939720pubmed:articleTitleTransgenic X. laevis embryos from eggs transplanted with nuclei of transfected cultured cells.lld:pubmed
pubmed-article:7939720pubmed:affiliationDepartment of Molecular and Cell Biology, University of California, Berkeley 94720.lld:pubmed
pubmed-article:7939720pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:7939720pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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