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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5185
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pubmed:dateCreated |
1994-11-21
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pubmed:abstractText |
Transgenic Xenopus laevis embryos were produced by transplantation of transfected cultured cell nuclei into unfertilized eggs. A Xenopus cell line, X-C, was stably transfected with plasmids containing a hygromycin-resistance gene and genes for either beta-galactosidase with a heat shock promoter or chloramphenicol acetyltransferase (CAT) with a muscle-specific actin promoter. Nuclei transplanted from these cells into unfertilized eggs directed development of embryos containing stably integrated copies of the plasmids in each cell. Transgenic embryos showed somite-specific expression of CAT and uniform expression of beta-galactosidase. Transgenic embryos produced by nuclear transplantation should be useful for testing the function of cloned genes in amphibian development.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
266
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
650-3
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7939720-Animals,
pubmed-meshheading:7939720-Animals, Genetically Modified,
pubmed-meshheading:7939720-Cell Line,
pubmed-meshheading:7939720-Cell Nucleus,
pubmed-meshheading:7939720-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:7939720-Cinnamates,
pubmed-meshheading:7939720-Drug Resistance,
pubmed-meshheading:7939720-Embryo, Nonmammalian,
pubmed-meshheading:7939720-Gene Expression,
pubmed-meshheading:7939720-Genes, Reporter,
pubmed-meshheading:7939720-Hygromycin B,
pubmed-meshheading:7939720-Nuclear Transfer Techniques,
pubmed-meshheading:7939720-Ovum,
pubmed-meshheading:7939720-Plasmids,
pubmed-meshheading:7939720-Promoter Regions, Genetic,
pubmed-meshheading:7939720-Transfection,
pubmed-meshheading:7939720-Xenopus laevis,
pubmed-meshheading:7939720-beta-Galactosidase
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pubmed:year |
1994
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pubmed:articleTitle |
Transgenic X. laevis embryos from eggs transplanted with nuclei of transfected cultured cells.
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pubmed:affiliation |
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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