Linked Life Data

7938942

Source:http://linkedlifedata.com/resource/pubmed/id/7938942

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-11-8
pubmed:abstractText
From a B. bovis gene sequence coding for a 60 kDa merozoite surface protein previously published, two sets of primers were designed for the Polymerase Chain Reaction (PCR) assay. Primer set BoF/BoR was used to prime Taq Polymerase DNA amplification of a 350 bp fragment of the target B. bovis DNA. Primer set BoFN/BoRN was used to prepare a PCR-synthesized, Digoxigenin-dUTP-labeled probe (291 bp) which would hybridize to a sequence within the PCR-amplified parasite target DNA. PCR amplification of target DNA obtained from in vitro-cultured B. bovis and nucleic acid hybridization of amplified product with the nonradioactive DNA probe showed that a 350 bp fragment could be detected when as little as 10 pg of genomic parasite DNA was utilized in the assay. A fragment of similar size was amplified from genomic DNA from four other B. bovis isolates but not from B. bigemina, Anaplasma marginale, or bovine leukocyte DNA. The PCR product was detected in blood samples containing approximately 3 B. bovis-infected erythrocytes (20 microliters of packed cells with a parasitemia of 0.000001%). By using the PCR/DNA probe assay, 16 out of 20 animals experimentally inoculated with B. bovis were detected positive, whereas no PCR product was observed in bovine blood samples collected from 20 B. bigemina-infected, and 20 uninfected cattle tested. The PCR-DNA probe assay was shown to be sensitive in detecting some cattle with B. bovis-chronic infection. The specificity and high analytical sensitivity of the test provides a valuable tool to apply in conducting epidemiological studies.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0187-4640
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
47-55
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Polymerase chain reaction-based diagnostic assay to detect cattle chronically infected with Babesia bovis.
pubmed:affiliation
Department of Veterinary Microbiology, UMC, Columbia, MO 65211.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't