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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1994-11-23
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pubmed:abstractText |
We have investigated the topology of the human beta 2-adrenergic receptor expressed in Escherichia coli, using the genetic method described by Beckwith and coworkers. We found that fusions with alkaline phosphatase beyond a certain point on the human beta 2-adrenergic receptor sequence were assembled into the bacterial membrane with the same topology as the human beta 2-adrenergic receptor in the mammalian membrane. The pattern that might have been expected on the basis of the topology of the human beta 2-adrenergic receptor in mammalian membranes was not reflected in the levels of alkaline phosphatase activity of the fusions occurring between the N-terminal region and positions close to the second external domain. Our data suggest that the correct positioning of the N terminus of the receptor depends on the presence of its C-terminal portions.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-1530939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-1544915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-1569545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-1593632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-1656450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-1702781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-1848865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-1915262,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-1938970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2119256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2164211,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2167830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2170984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2175604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2200169,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2205394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2359127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2404939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2474546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2551889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2660110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2821000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2845411,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-2999794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-3001640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-3025863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-3038163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-3529391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-3915782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-6339478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-6788377,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-7108955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-7694597,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937986-8419303
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10521-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7937986-Alkaline Phosphatase,
pubmed-meshheading:7937986-Animals,
pubmed-meshheading:7937986-Cell Membrane,
pubmed-meshheading:7937986-Cloning, Molecular,
pubmed-meshheading:7937986-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7937986-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7937986-Escherichia coli,
pubmed-meshheading:7937986-Immunoblotting,
pubmed-meshheading:7937986-Isoproterenol,
pubmed-meshheading:7937986-Mammals,
pubmed-meshheading:7937986-Methionine,
pubmed-meshheading:7937986-Models, Structural,
pubmed-meshheading:7937986-Molecular Sequence Data,
pubmed-meshheading:7937986-Plasmids,
pubmed-meshheading:7937986-Protein Conformation,
pubmed-meshheading:7937986-Receptors, Adrenergic, beta-1,
pubmed-meshheading:7937986-Recombinant Fusion Proteins,
pubmed-meshheading:7937986-Recombinant Proteins,
pubmed-meshheading:7937986-Sulfur Radioisotopes
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pubmed:year |
1994
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pubmed:articleTitle |
Topological analysis of the human beta 2-adrenergic receptor expressed in Escherichia coli.
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pubmed:affiliation |
EniChem, Rome, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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