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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
21
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pubmed:dateCreated |
1994-11-10
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pubmed:abstractText |
Immunization with irradiated sporozoites protects animals and humans against malaria, and the circumsporozoite protein is a target of this protective immunity. We now report that adjuvant-free intramuscular injection of mice with plasmid DNA encoding the Plasmodium yoelii circumsporozoite protein induced higher levels of antibodies and cytotoxic T lymphocytes against the P. yoelii circumsporozoite protein than did immunization with irradiated sporozoites. Mice immunized with this vaccine had an 86% reduction in liver-stage parasite burden after challenge with 5 x 10(5) sporozoites (> 10(5) median infectious doses). Eighteen (68%) of 28 mice that received two or three doses of vaccine were protected against challenge with 10(2) sporozoites, and the protection was dependent on CD8+ T cells. These studies demonstrate the utility of plasmid DNA immunization against a nonviral infection. By obviating the requirement for peptide synthesis, expression and purification of recombinant proteins, and adjuvants, this method of immunization provides an important alternative for rapid identification of protective B- and T-cell epitopes and for construction of vaccines to prevent malaria and other infectious diseases.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-1323900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-1501644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-1517574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-1545867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-1689762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-1710037,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-1716146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-1827210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-1988490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-2094580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-2094592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-2456467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-2472543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-2524877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-2963334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-3102479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-3325624,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-3479809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-3530501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-39127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-7679428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-7685119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-8212843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-8265577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-8350420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-8399489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-8456302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937907-8483929
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
91
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9866-70
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7937907-Animals,
pubmed-meshheading:7937907-Antibodies, Protozoan,
pubmed-meshheading:7937907-Antigens, Protozoan,
pubmed-meshheading:7937907-CD8-Positive T-Lymphocytes,
pubmed-meshheading:7937907-Cytotoxicity, Immunologic,
pubmed-meshheading:7937907-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7937907-Female,
pubmed-meshheading:7937907-Humans,
pubmed-meshheading:7937907-Immunization,
pubmed-meshheading:7937907-Liver,
pubmed-meshheading:7937907-Malaria,
pubmed-meshheading:7937907-Mice,
pubmed-meshheading:7937907-Mice, Inbred BALB C,
pubmed-meshheading:7937907-Plasmids,
pubmed-meshheading:7937907-Plasmodium yoelii,
pubmed-meshheading:7937907-Protozoan Proteins,
pubmed-meshheading:7937907-Time Factors
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pubmed:year |
1994
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pubmed:articleTitle |
Protection against malaria by immunization with plasmid DNA encoding circumsporozoite protein.
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pubmed:affiliation |
Malaria Program, Naval Medical Research Institute, Bethesda, MD 20889-5607.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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