Linked Life Data

7937330

Source:http://linkedlifedata.com/resource/pubmed/id/7937330

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-10-28
pubmed:abstractText
Pituitary cells from 14-day-old rats were separated by unit gravity velocity sedimentation, and a highly enriched population of gonadotrophs was established in reaggregate cell culture in serum-free and serum albumin-free defined culture media. The medium conditioned by these aggregates was concentrated, ultrafiltrated, and the concentrated substances consecutively separated by two reversed-phase HPLC steps, a gel filtration step and an additional reversed-phase purification step. Two peptides could be isolated that displayed an N-terminal amino acid sequence identical to fragments of rat presecretogranin II: one was cleaved at the dibasic amino acid residues K182-R183 and the other at the dibasic amino acid residues K569-R570. The latter peptide was completely purified and separated into three variants with the same N-terminal amino acid sequence. From an analysis by electrospray ionization mass spectrometry, it was deduced that the three forms extended up to amino acid residue 612 (A), which is, in presecretogranin II, also flanked by two basic amino acids (K613R614). Two variant forms had a molecular mass that was 17 Da higher. The present data support the hypothesis that secretogranin II is a precursor of putative regulatory peptides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0196-9781
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
537-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Isolation of two peptides from rat gonadotroph-conditioned medium displaying an amino acid sequence identical to fragments of secretogranin II.
pubmed:affiliation
Laboratory of Cell Pharmacology, Catholic University of Leuven, Belgium.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't