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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1994-11-16
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pubmed:abstractText |
Recent studies with 8-oxodeoxyguanosine triphosphate (8-oxodGTP) have suggested that incorporation of oxidized nucleotides from the precursor pool into DNA may have deleterious effects. Here we show that 5-hydroxydeoxycytosine triphosphate (5-OHdCTP) and 5-hydroxydeoxyuridine triphosphate (5-OHdUTP) are more efficient substrates than 8-oxodGTP for Escherichia coli DNA polymerase I Klenow fragment lacking proofreading activity, while 8-oxodeoxyadenosine triphosphate (8-oxodGTP, 5-OHdCTP can mispair with dA in DNA but with lower efficiency. Since the 5-hydroxypyrimidines are present in normal and oxidized cellular DNA in amounts similar to the 8-oxopurines, these data suggest that enzymatic mechanisms might exist for removing them from the DNA precursor pools.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1309939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1328155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1332067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1383774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-14289336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1461734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1495996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1565629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1565630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1650737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1650738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1730583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1826106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1850508,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1947004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1947005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1975603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-1992344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-2052015,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-2052552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-2223758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-2261442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-2602371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-2966755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-3003798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-3034813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-3088546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-3413108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-3667598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-3769133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-4171891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-5328724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-6286632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-6326053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-6701097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-7047496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-7683083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-8127657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-8161527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-8483909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937115-8485138
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3930-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7937115-Base Sequence,
pubmed-meshheading:7937115-DNA,
pubmed-meshheading:7937115-DNA Polymerase I,
pubmed-meshheading:7937115-Deoxyadenine Nucleotides,
pubmed-meshheading:7937115-Deoxycytosine Nucleotides,
pubmed-meshheading:7937115-Deoxyguanine Nucleotides,
pubmed-meshheading:7937115-Deoxyuracil Nucleotides,
pubmed-meshheading:7937115-Kinetics,
pubmed-meshheading:7937115-Molecular Sequence Data,
pubmed-meshheading:7937115-Templates, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
5-Hydroxypyrimidine deoxynucleoside triphosphates are more efficiently incorporated into DNA by exonuclease-free Klenow fragment than 8-oxopurine deoxynucleoside triphosphates.
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pubmed:affiliation |
Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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