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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
18
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pubmed:dateCreated |
1994-11-3
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pubmed:abstractText |
We report a method to analyse multiple samples by fluorescence in situ hybridisation on a single glass microscope slide. Wells were formed in which independent hybridisation reactions could proceed by sealing a silicon rubber gasket to the slide. In the largest format tested, different probes were hybridised simultaneously by applying them directly from a 96-well microtitre dish which was inverted on a glass plate. This technique will increase the rate of analysis of multiple probes against a standard set of chromosomes and could also be used to analyse different karyotypes using a panel of probes such as single chromosome paints during a single operation. It should be useful for both chromosomal mapping projects and screening for chromosome abnormalities in clinical diagnostic laboratories.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-1301167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-1303232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-1359640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-1384055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-1406950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-1559699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-16593771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-1691830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-1741394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-2034658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-2294592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-2405397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-2591960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-2973607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-8081355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-8106079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-8259213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937078-8284234
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
22
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3689-92
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
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pubmed:year |
1994
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pubmed:articleTitle |
Fluorescence in situ hybridisation of multiple probes on a single microscope slide.
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pubmed:affiliation |
Department of Biochemistry, Oxford University, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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