Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1994-11-18
pubmed:abstractText
To study the transcriptional regulation of the liver gluconeogenic phenotype, the underdifferentiated mouse Hepa-1c1c7 (Hepa) hepatoma cell line was used. These cells mimicked the fetal liver by appreciably expressing the alpha-fetoprotein and albumin genes but not the phosphoenolpyruvate carboxykinase (PEPCK) gene. Unlike the fetal liver, however, Hepa cells failed to express the early-expressed factors hepatocyte nuclear factor 1 alpha (HNF-1 alpha) and HNF-4 and the late-expressed factor C/EBP alpha, thereby providing a suitable system for examining possible cooperation between these factors in the transcriptional regulation of the PEPCK gene. Transient transfection assays of a chimeric PEPCK-chloramphenicol acetyltransferase construct showed a residual PEPCK promoter activity in the Hepa cell line, which was slightly stimulated by cotransfection with a single transcription factor from either the C/EBP family or HNF-1 alpha but not at all affected by cotransfection of HNF-4. In contrast, cotransfection of the PEPCK construct with members from the C/EBP family plus HNF-1 alpha resulted in a synergistic stimulation of the PEPCK promoter activity. This synergistic effect depended on the presence in the PEPCK promoter region of the HNF-1 recognition sequence and on the presence of two C/EBP recognition sequences. The results demonstrate a requirement for coexistence and cooperation between early and late liver-enriched transcription factors in the transcriptional regulation of the PEPCK gene. In addition, the results suggest redundancy between members of the C/EBP family of transcription factors in the regulation of PEPCK gene expression.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-106390, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1312668, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1324404, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1330322, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1333043, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1337626, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1363228, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-14143233, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1545785, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1545820, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1547942, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1649701, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1655770, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1656224, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1673926, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1685988, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1687651, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1764997, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1776173, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1832337, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1889088, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-1934061, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2014251, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2055492, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2062840, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2147222, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2166335, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2265613, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2279702, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2294400, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2325658, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2342460, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2344618, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2388623, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2461942, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2542317, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2601701, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2792758, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2847161, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2850495, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-2919162, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-291970, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-3015903, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-3026224, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-3029767, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-3323813, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-3431441, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-3670292, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-36985, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-3779841, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-388439, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-4291934, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-4504350, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-518835, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-6170978, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-6182563, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-6304730, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-6502710, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-6960240, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-8093246, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-8269513, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-8288579, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-8288597, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-8306889, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-8344962, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-8381439, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-8449898, http://linkedlifedata.com/resource/pubmed/commentcorrection/7935427-88451
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Basic Helix-Loop-Helix Leucine..., http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1, http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-alpha, http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 1-beta, http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 4, http://linkedlifedata.com/resource/pubmed/chemical/Hnf1a protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Hnf1a protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Hnf1b protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoenolpyruvate Carboxykinase..., http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Tcfl4 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0270-7306
pubmed:author
More...