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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0022369,
umls-concept:C0027836,
umls-concept:C0040649,
umls-concept:C0086418,
umls-concept:C0086860,
umls-concept:C0185117,
umls-concept:C0205087,
umls-concept:C0597065,
umls-concept:C1280500,
umls-concept:C1823882,
umls-concept:C2911684
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pubmed:issue |
11
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pubmed:dateCreated |
1994-11-17
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pubmed:abstractText |
We have isolated a partial recombinant cDNA clone from a HeLa expression library which encodes a protein capable of binding to the central region of the human neurotropic JC virus (JCV) enhancer/promoter, termed the B region. Sequence analysis revealed a complete homology of the partial cDNA clone to the N-terminal region, of a previously described DNA-binding protein, termed YB-1. Band shift analyses have indicated that the bacterially produced YB-1 interacts specifically with the double-stranded B oligonucleotide as well as the corresponding single-stranded DNA fragment representing the early promoter sequence. Further analysis indicated that the YB-1 protein binds specifically to the C/T-rich sequence of the B domain, which is located in close proximity to the TATA box within the virus enhancer/promoter. Results from cotransfection experiments demonstrated that the full-length (YB-1) but not the partial cDNA enhances expression of the JCV late (JCVL) promoter in glial cells. Cointroduction into glial cells of a recombinant expressing the YB-1 and JCVL deletion mutants indicated that removal of the C/T-rich sequence of the B domain reduces the level of activation of the virus promoter by YB-1. Further cotransfection experiments revealed that the virus transactivating protein T antigen appears to diminish the ability of YB-1 to activate JCVL gene expression. RNA studies indicated that YB-1 is expressed in several cell types and tissues. Examination of YB-1 RNA from mouse brain at various stages of development revealed high levels of YB-1 RNA at early stages of development and lower levels at all subsequent developmental stages.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1310438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1331525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1622927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1622930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1714899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1729676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1738588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1891365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1923758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1961761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1967130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-1986360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2111709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2142689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2166044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2247479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2300056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2404279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2504497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2535750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2540170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2541545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2768236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2841118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2845416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-2977358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-3035549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-3061875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-3114745,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-3174636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-3289117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-3398920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-3607873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-3757032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-4571704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-4705382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-6086957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-6095453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-8031301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933155-8388103
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral, Tumor,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NFI Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Y-Box-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/YBX1 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7637-43
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7933155-Humans,
pubmed-meshheading:7933155-Nucleoproteins,
pubmed-meshheading:7933155-Neuroglia,
pubmed-meshheading:7933155-Base Sequence,
pubmed-meshheading:7933155-HeLa Cells,
pubmed-meshheading:7933155-Genes, Viral,
pubmed-meshheading:7933155-Molecular Sequence Data,
pubmed-meshheading:7933155-Nuclear Proteins,
pubmed-meshheading:7933155-Transcription, Genetic,
pubmed-meshheading:7933155-Antigens, Viral, Tumor,
pubmed-meshheading:7933155-Promoter Regions, Genetic,
pubmed-meshheading:7933155-DNA-Binding Proteins,
pubmed-meshheading:7933155-DNA, Complementary
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