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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1994-11-17
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pubmed:abstractText |
Previously we demonstrated an inhibitory effect of interleukin-4 (IL-4) on establishment of human immunodeficiency virus type 1 (HIV-1) infection in primary macrophages. The reported similarities between the biological effects of IL-4 and IL-10 prompted us to study the effect of IL-10 on HIV-1 replication. Treatment of primary macrophages with IL-10 resulted in inhibition of HIV-1 infection. This inhibitory effect was specific for macrophages, since IL-10 did not interfere with HIV-1 replication in primary T cells. Semiquantitative PCR analysis excluded an inhibitory effect of IL-10 on virus entry and reverse transcription. Effects of IL-10 on HIV-1 long terminal repeat-driven chloramphenicol acetyltransferase activity also could not be demonstrated in a transient expression system in primary derived macrophages. In agreement with this, Northern (RNA) blot analysis demonstrated equal amounts of viral RNA species irrespective of IL-10 treatment, also excluding an inhibitory effect on elongation of virus transcription. Monocyte-derived macrophages (MDM) treated with IL-10 after HIV-1 inoculation showed accumulation of apparently mature p24 protein suggestive of an inhibitory effect at the level of virus assembly. IL-10 treatment of MDM prior to HIV-1 inoculation did not result in accumulation of p24 protein. Immunoblot analysis indeed showed the absence of mature p24 and gp120 but accumulation of the Pr53 gag-encoded protein in HIV-1-inoculated, IL-10-pretreated MDM, suggesting an inhibitory effect at the level of protein processing. A combination of IL-4 and IL-10 resulted in a cumulative inhibitory effect on HIV-1 replication in MDM. The recent observation that in the course of HIV-1 infection a shift occurs in the production of IL-2/gamma interferon toward enhanced IL-4 and IL-10 production and the reported shift from preferential macrophage-tropic towards preferential T-cell-tropic HIV-1 variants with progression of disease suggest that cytokines have an important role in the in vivo regulation of HIV-1 tropism.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-1556179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-1655948,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-1738194,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-1940799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-1949706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-1972163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-1985204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2104921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2119829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2191685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2193094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2207324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2331748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2466937,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2472701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2530302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2665081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2665943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2825027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2830675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2849111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2990040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-2999980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-3014648,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-3031512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-3047875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-3123712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-6200935,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-7082846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-7687627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-8023142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-8096068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-8098942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-8101865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-8113410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-8235617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7933078-8450057
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6967-75
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7933078-Base Sequence,
pubmed-meshheading:7933078-Cells, Cultured,
pubmed-meshheading:7933078-Dose-Response Relationship, Drug,
pubmed-meshheading:7933078-HIV Core Protein p24,
pubmed-meshheading:7933078-HIV-1,
pubmed-meshheading:7933078-Humans,
pubmed-meshheading:7933078-Interleukin-10,
pubmed-meshheading:7933078-Interleukin-4,
pubmed-meshheading:7933078-Macrophages,
pubmed-meshheading:7933078-Molecular Sequence Data,
pubmed-meshheading:7933078-Monocytes,
pubmed-meshheading:7933078-Transcription, Genetic,
pubmed-meshheading:7933078-Virus Replication
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pubmed:year |
1994
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pubmed:articleTitle |
Interference of interleukin-10 with human immunodeficiency virus type 1 replication in primary monocyte-derived macrophages.
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pubmed:affiliation |
Department of Clinical Viro-Immunology, Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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