Linked Life Data

7932871

Source:http://linkedlifedata.com/resource/pubmed/id/7932871

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-11-23
pubmed:abstractText
The neuropeptide cholecystokinin (CCK) is involved in the regulation of female, but not male, reproductive behavior. In both sexes, estrogen regulates the expression of CCK in adulthood within the bed nucleus of the stria terminalis and medial amygdaloid nucleus. These areas are parts of an interconnected limbic system-hypothalamic circuit, the development of which is influenced by estrogen during the early postnatal period. This is the same period during which central nervous system (CNS) expression of CCK is dramatically increased, suggesting that the male and female patterns of CCK expression may be the result of early postnatal exposure to estrogen. In the present experiment, the expression of preprocholecystokinin (pCCK) mRNA was determined by in situ hybridization with an isotopically labeled pCCK complementary RNA and emulsion autoradiography in animals whose neonatal and adult gonadal steroid levels had been manipulated. The number of pCCK-expressing cells in animals that were gonadectomized as adults was determined by neonatal estrogen, but stimulation with steroids in adulthood induced a similar number of pCCK-expressing cells in both sexes in the medial amygdala and bed nucleus of the stria terminalis. Neonatal treatment of females with estrogen or testosterone, followed by ovariectomy in adulthood, eliminated the sex difference in pCCK mRNA expression. Males treated neonatally with the aromatase inhibitor androstenedione (to block metabolism of testosterone to estrogen) and orchidectomized in adulthood had a level of pCCK mRNA expression that was similar to that of ovariectomized females. These data suggest that, during neonatal development, estrogen determines the constitutive expression of pCCK mRNA in the medial amygdala and bed nucleus of the stria terminalis, resulting in higher levels of pCCK mRNA expression in males than in females. However, exogenous gonadal steroids induce the same levels of pCCK mRNA expression in adult females, indicating that the levels of gonadal steroids and the patterns of their secretion are the predominant influences on the sexually dimorphic adult levels of pCCK mRNA expression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
386-98
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7932871-Aging, pubmed-meshheading:7932871-Amygdala, pubmed-meshheading:7932871-Animals, pubmed-meshheading:7932871-Animals, Newborn, pubmed-meshheading:7932871-Autoradiography, pubmed-meshheading:7932871-Cholecystokinin, pubmed-meshheading:7932871-Female, pubmed-meshheading:7932871-Gonadal Steroid Hormones, pubmed-meshheading:7932871-Hypothalamus, pubmed-meshheading:7932871-In Situ Hybridization, pubmed-meshheading:7932871-Limbic System, pubmed-meshheading:7932871-Male, pubmed-meshheading:7932871-Orchiectomy, pubmed-meshheading:7932871-Ovariectomy, pubmed-meshheading:7932871-Pons, pubmed-meshheading:7932871-Protein Precursors, pubmed-meshheading:7932871-RNA, Messenger, pubmed-meshheading:7932871-RNA Probes, pubmed-meshheading:7932871-Rats, pubmed-meshheading:7932871-Sex Characteristics
pubmed:year
1994
pubmed:articleTitle
Gonadal steroid control of preprocholecystokinin mRNA expression in the limbic-hypothalamic circuit: comparison of adult with neonatal steroid treatments.
pubmed:affiliation
Department of Anatomy and Cell Biology, UCLA School of Medicine.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.