Linked Life Data

7930731

Source:http://linkedlifedata.com/resource/pubmed/id/7930731

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1994-11-1
pubmed:abstractText
The presence of outer surface protein A (OspA) as a marker for Borrelia burgdorferi in Ixodes scapularis ticks was monitored with an OspA antigen-capture ELISA. The OspA ELISA, with a sensitivity of 30 spirochetes (8 fg), was not affected by the presence of either recently blood-fed or flat tick homogenates. Median spirochete equivalent levels as high as 16,000 in larvae, 55,000 in attached nymphs, and 10,000 in unfed adults were observed. Estimates of OspA doubling times ranged from a maximum of 140 h in larvae to as short as 17.5 h during nymphal attachment to the host. Spirochete equivalents in nymphs fell by 3 x 10(4) spirochete equivalents (54% of total OspA) in the last 12 h of attachment to mice but rose after detachment. Each OspA-positive I. scapularis nymph, regardless of spirochete equivalent density or length of attachment, successfully transmitted B. burgdorferi to a mouse.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1899
pubmed:author
pubmed:issnType
Print
pubmed:volume
170
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
883-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Quantitation of the Borrelia burgdorferi outer surface protein A in Ixodes scapularis: fluctuations during the tick life cycle, doubling times, and loss while feeding.
pubmed:affiliation
Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, Fort Collins, Colorado 80522.
pubmed:publicationType
Journal Article