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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1994-11-10
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pubmed:abstractText |
Renal ischemia results in both a profound fall in cellular ATP and a rapid induction of the 70 kD heat-shock protein family, HSP-70. The present studies examined the relationship between cellular ATP and induction of the stress response in renal cortex. Cellular ATP, continuously monitored by in vivo 31P-NMR spectroscopy, was reduced and maintained at specific, stable levels in renal cortex by partial aortic occlusion for 45 min. Activation of heat-shock transcription factor (HSF) was detected by gel retardation assay and transcription was confirmed by Northern analysis. Activation of HSF was not present, and HSP-70 mRNA induction did not occur when ATP levels were maintained above 60% preocclusion (control) levels. Reduction in cortical ATP levels to 35-50% preocclusion values resulted in HSF activation and low-level expression of inducible HSP-70 mRNA. Cellular ATP of 20-25% control values resulted in a greater level of HSF activation and subsequent HSP-70 mRNA elaboration. HSF was activated at the end of 15 min of total occlusion. The studies indicate that a 50% reduction in cellular ATP in the renal cortex must occur before the stress response is detectable, that reduction of ATP below 25% control levels produces a more vigorous response, and that reperfusion is not required for initiation of a heat-shock response in the kidney. Cellular ATP, or the metabolic consequences associated with ATP depletion, may be a threshold factor for initiation of a stress response in the kidney.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1297327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1405356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1443167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1546322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1569208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1607378,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1607379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1628823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1658790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1696723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1829527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1936996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-1986252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-2012203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-2018972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-2062034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-2203539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-2320006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-3198647,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-3211126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-7970927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-8214091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-8334697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-8378118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-8416735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-8451637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-8464927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7929828-8479154
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9738
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
94
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1518-23
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7929828-Adenosine Triphosphate,
pubmed-meshheading:7929828-Animals,
pubmed-meshheading:7929828-Constriction, Pathologic,
pubmed-meshheading:7929828-DNA,
pubmed-meshheading:7929828-HSP70 Heat-Shock Proteins,
pubmed-meshheading:7929828-Hydrogen-Ion Concentration,
pubmed-meshheading:7929828-Ischemia,
pubmed-meshheading:7929828-Kidney Cortex,
pubmed-meshheading:7929828-Male,
pubmed-meshheading:7929828-Protein Binding,
pubmed-meshheading:7929828-RNA, Messenger,
pubmed-meshheading:7929828-Rats,
pubmed-meshheading:7929828-Rats, Sprague-Dawley,
pubmed-meshheading:7929828-Transcriptional Activation
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pubmed:year |
1994
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pubmed:articleTitle |
Activation of heat-shock transcription factor by graded reductions in renal ATP, in vivo, in the rat.
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pubmed:affiliation |
Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut 06510.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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