| pubmed-article:7927808 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7927808 | lifeskim:mentions | umls-concept:C0034861 | lld:lifeskim |
| pubmed-article:7927808 | lifeskim:mentions | umls-concept:C0006304 | lld:lifeskim |
| pubmed-article:7927808 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:7927808 | lifeskim:mentions | umls-concept:C0524637 | lld:lifeskim |
| pubmed-article:7927808 | lifeskim:mentions | umls-concept:C0035553 | lld:lifeskim |
| pubmed-article:7927808 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:7927808 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:7927808 | lifeskim:mentions | umls-concept:C1539707 | lld:lifeskim |
| pubmed-article:7927808 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:7927808 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:7927808 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:7927808 | pubmed:dateCreated | 1994-11-22 | lld:pubmed |
| pubmed-article:7927808 | pubmed:abstractText | The Brucella abortus L7/L12 ribosomal gene was amplified by PCR and subcloned into the prokaryotic expression vector pMAL-c2. Escherichia coli DH5 alpha was transformed with the pMAL-L7/L12 construct, and gene expression was induced by IPTG (isopropyl-beta-D-thiogalactopyranoside). The resulting fusion protein was purified by affinity chromatography and confirmed by Western blot (immunoblot) analysis using an anti-maltose-binding protein antibody. Additionally, purified recombinant L7/L12 protein induced T-lymphocyte proliferation of B. abortus-primed bovine peripheral blood mononuclear cells. Phenotypic analysis of the proliferating cell population demonstrated an increase in the percentage of CD4+ T lymphocytes when peripheral blood mononuclear cells were cultured with recombinant L7/L12 compared with cells cultured in medium alone. Subcloning and expression of a B. abortus gene encoding a previously demonstrated immunodominant protein for bovine lymphocytes are important steps in selecting Brucella proteins that have potential as a component of a genetically engineered candidate vaccine. | lld:pubmed |
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| pubmed-article:7927808 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7927808 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7927808 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7927808 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7927808 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7927808 | pubmed:issn | 0019-9567 | lld:pubmed |
| pubmed-article:7927808 | pubmed:author | pubmed-author:SplitterG AGA | lld:pubmed |
| pubmed-article:7927808 | pubmed:author | pubmed-author:OliveiraS CSC | lld:pubmed |
| pubmed-article:7927808 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7927808 | pubmed:volume | 62 | lld:pubmed |
| pubmed-article:7927808 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7927808 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7927808 | pubmed:pagination | 5201-4 | lld:pubmed |
| pubmed-article:7927808 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7927808 | pubmed:meshHeading | pubmed-meshheading:7927808-... | lld:pubmed |
| pubmed-article:7927808 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7927808 | pubmed:articleTitle | Subcloning and expression of the Brucella abortus L7/L12 ribosomal gene and T-lymphocyte recognition of the recombinant protein. | lld:pubmed |
| pubmed-article:7927808 | pubmed:affiliation | Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison 53706. | lld:pubmed |
| pubmed-article:7927808 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7927808 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:7927808 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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