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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1994-11-22
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pubmed:abstractText |
The Brucella abortus L7/L12 ribosomal gene was amplified by PCR and subcloned into the prokaryotic expression vector pMAL-c2. Escherichia coli DH5 alpha was transformed with the pMAL-L7/L12 construct, and gene expression was induced by IPTG (isopropyl-beta-D-thiogalactopyranoside). The resulting fusion protein was purified by affinity chromatography and confirmed by Western blot (immunoblot) analysis using an anti-maltose-binding protein antibody. Additionally, purified recombinant L7/L12 protein induced T-lymphocyte proliferation of B. abortus-primed bovine peripheral blood mononuclear cells. Phenotypic analysis of the proliferating cell population demonstrated an increase in the percentage of CD4+ T lymphocytes when peripheral blood mononuclear cells were cultured with recombinant L7/L12 compared with cells cultured in medium alone. Subcloning and expression of a B. abortus gene encoding a previously demonstrated immunodominant protein for bovine lymphocytes are important steps in selecting Brucella proteins that have potential as a component of a genetically engineered candidate vaccine.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-1350274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-1587614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-1730920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-1904713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-2423591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-2472288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-2509359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-2528463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-2660100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-2843437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-3110273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-3126823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-3133283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-3514442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-3518022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-3533100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-3894225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-6356268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-6411853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-8112833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-8125331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-8143124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-8225607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-8310661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-8359926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927808-8514387
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
62
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5201-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7927808-Animals,
pubmed-meshheading:7927808-Base Sequence,
pubmed-meshheading:7927808-Brucella abortus,
pubmed-meshheading:7927808-Cattle,
pubmed-meshheading:7927808-Cloning, Molecular,
pubmed-meshheading:7927808-DNA Primers,
pubmed-meshheading:7927808-Genes, Bacterial,
pubmed-meshheading:7927808-Molecular Sequence Data,
pubmed-meshheading:7927808-Recombinant Proteins,
pubmed-meshheading:7927808-Ribosomal Proteins,
pubmed-meshheading:7927808-T-Lymphocytes
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pubmed:year |
1994
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pubmed:articleTitle |
Subcloning and expression of the Brucella abortus L7/L12 ribosomal gene and T-lymphocyte recognition of the recombinant protein.
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pubmed:affiliation |
Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison 53706.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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