Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-10-21
pubmed:abstractText
Silica (SiO2) or related substances such as silicone ([-R2Si-O-]n), which is used in plastic surgery, or asbestos (e.g. chrysotile; 3MgO.2SiO2.H2O) have 'adjuvant effects'. In a study of scleroderma patients in Germany more than 78% had experienced exposure to silicate dust. T-cell receptor (TcR) V beta gene analysis on CD4- CD8- double-negative alpha beta T cells from scleroderma patients, using polymerase chain reaction (PCR), showed that certain V beta genes, V beta 5, V beta 7 and V beta 17, were predominantly expressed in the cells. We found that certain V beta repertoires, V beta 5.3 and V beta 6.7, were predominantly expressed on fractionated T cells with a high Ca2+ level that had been stimulated by chrysotile in vitro. The intracellular Ca2+ level in human peripheral blood mononuclear cells (PBMC) increased after incubation with silica or chrysotile. Interleukin-2 (IL-2) release from PMBC also rose significantly with chrysotile stimulation, but no change was observed when major histocompatibility complex (MHC) class II DP/DR positive cells were depleted. Therefore, our results support the possibility that silicate acts as a superantigen.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-1324277, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-1382835, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-145543, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-1500856, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-1531348, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-1660155, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-1671839, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-1680703, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-1681539, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-2118929, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-2164711, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-3097874, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-3280186, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-7678036, http://linkedlifedata.com/resource/pubmed/commentcorrection/7927506-7678619
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0019-2805
pubmed:author
pubmed:issnType
Print
pubmed:volume
82
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
332-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Polyclonal human T-cell activation by silicate in vitro.
pubmed:affiliation
Department of Hygiene, Kawasaki Medical School, Kurashiki, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't