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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-10-21
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pubmed:abstractText |
We have investigated several aspects of gamma delta T cells in sheep. gamma delta T cells of sheep express a unique transmembrane protein termed T19 but lack the expression of particular cell-surface molecules such as CD2, CD4 and CD8 which are typically associated with alpha beta T cells. The majority of gamma delta T cells isolated from animals of all ages examined lacked the expression of CD45RA. A faster rate of activation by gamma delta T cells compared to either CD4 or CD8 T cells was seen in the time-course of IL-2 receptor alpha chain (CD25) cell-surface expression. All gamma delta T cells expressed the CD25 protein within 8 hr of activation whereas the majority of CD4 or CD8 T cells did not express CD25 until 24 hr post-concanavalin A (Con A) stimulation. This difference in the rate of expression of activation molecules was not restricted to CD25, as a similar trend was seen with cell-surface expression of major histocompatibility complex (MHC) class II molecules. We have used the distinct phenotypic profile of ovine gamma delta T cells to purify these cells by positive selection via the T19 molecule to assess their in vitro proliferative response to various antigens. Routinely, cell populations comprising more than 93% gamma delta T cells with yields of approximately 55% were obtained. Purified gamma delta T cells were capable of responding to Mycobacterium tuberculosis antigen in a primary and secondary in vitro proliferation assay and to ovalbumin in a secondary response. Ovine gamma delta T cells showed little, if any, proliferative response to allogeneic stimulator cells.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-1358625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-1431105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-1531636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-1634253,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-1716171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-1826600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-1832874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-1832950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-1900055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-1971793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2111341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2141552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2143170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2147918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2307933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2379238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2443855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2455897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2462499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2466078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2477705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2528206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2528463,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2697682,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-2978457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-3081353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-3110786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-3290379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-3309677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-3894225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927494-8440261
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
229-37
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7927494-Animals,
pubmed-meshheading:7927494-Antigens,
pubmed-meshheading:7927494-Antigens, CD,
pubmed-meshheading:7927494-Antigens, CD45,
pubmed-meshheading:7927494-Cell Division,
pubmed-meshheading:7927494-Cell Separation,
pubmed-meshheading:7927494-Cells, Cultured,
pubmed-meshheading:7927494-Concanavalin A,
pubmed-meshheading:7927494-Female,
pubmed-meshheading:7927494-Lymphocyte Activation,
pubmed-meshheading:7927494-Male,
pubmed-meshheading:7927494-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:7927494-Receptors, Interleukin-2,
pubmed-meshheading:7927494-Sheep,
pubmed-meshheading:7927494-T-Lymphocyte Subsets
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pubmed:year |
1994
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pubmed:articleTitle |
Antigen recognition and activation of ovine gamma delta T cells.
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pubmed:affiliation |
Department of Veterinary Pathology, University of Edinburgh.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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