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rdf:type |
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lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0010823,
umls-concept:C0016030,
umls-concept:C0019721,
umls-concept:C0022687,
umls-concept:C0041904,
umls-concept:C0086418,
umls-concept:C0162493,
umls-concept:C0205148,
umls-concept:C0205263,
umls-concept:C0205725,
umls-concept:C0221284,
umls-concept:C0456387,
umls-concept:C0524828,
umls-concept:C0567416,
umls-concept:C0683598
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pubmed:issue |
2
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pubmed:dateCreated |
1994-10-21
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pubmed:abstractText |
We have investigated the susceptibility of human foreskin fibroblast (HFF) monolayers infected at low level with human cytomegalovirus (HCMV) strain AD 169, or a clinical HCMV isolate, to lysis mediated by interleukin-2(IL-2)-activated killer cells (LAK). HFF cultures inoculated with a multiplicity of infection (MOI) dose of 0.001-0.01 had significantly decreased susceptibility to lysis by IL-2-activated non-adherent blood cells (PNAC) or purified CD56+ cells in comparison to mock-infected cultures. By 12h post incubation HFF cultures showed diminished susceptibility to LAK cell cytotoxicity when HFF cultures were incubated with HCMV (MOI 0.01) or interferon-beta (IFN-beta; 100 U/ml). Cytofluorometric analysis of HCMV-infected HFF cultures showed a modulation of HLA class I expression on single cells 3 days post-infection, namely, segregation of the cells in low- and high-density HLA class I-expressing cells depended on the dose of HCMV inoculum. However, up-regulation of HLA class I surface molecules was not significantly enhanced 12 h post-incubation with HCMV inoculum or IFN-beta. Anti-IFN-beta antibodies prevented both the development of the resistance and the increase of HLA class I expression in infected HFF cultures. In summary, the comparison of HLA class I expression and the LAK susceptibility of HCMV-infected HFF cultures may lead to the following conclusions: IFN-beta mediates the protection of neighbouring uninfected fibroblasts, but the modulation of HLA class I expression on uninfected cells does not correlate with the diminished susceptibility.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-1315587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-1325654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-1328494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-1421956,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-1500711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-1545133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-1654370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-1658363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-1686171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-1851136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2021423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2054113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2154697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2172831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2201309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2413125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2440628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2446012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2453457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2463268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2543925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2784569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-2827039,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-3014033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-3029305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-3493296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-3726459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-5637478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-6161162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-6190939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-6199426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-7451975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-7683316,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-8198451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-8344707,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-8384753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-8394407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-8432986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7927485-8466640
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
171-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7927485-Cells, Cultured,
pubmed-meshheading:7927485-Cytomegalovirus Infections,
pubmed-meshheading:7927485-Cytotoxicity, Immunologic,
pubmed-meshheading:7927485-Fibroblasts,
pubmed-meshheading:7927485-Flow Cytometry,
pubmed-meshheading:7927485-Histocompatibility Antigens Class I,
pubmed-meshheading:7927485-Humans,
pubmed-meshheading:7927485-Interferon-beta,
pubmed-meshheading:7927485-Interleukin-2,
pubmed-meshheading:7927485-Killer Cells, Lymphokine-Activated,
pubmed-meshheading:7927485-Kinetics,
pubmed-meshheading:7927485-Up-Regulation
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pubmed:year |
1994
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pubmed:articleTitle |
Low-dose human cytomegalovirus infection of human fibroblast cultures induces lymphokine-activated killer cell resistance: interferon-beta-mediated target cell protection does not correlate with up-regulation of HLA class I surface molecules.
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pubmed:affiliation |
Department of Medical Virology and Epidemiology of Viral Diseases, Hygiene-Institut, University of Tübingen, Germany.
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pubmed:publicationType |
Journal Article
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