Linked Life Data

7926837

Source:http://linkedlifedata.com/resource/pubmed/id/7926837

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-11-18
pubmed:databankReference
pubmed:abstractText
Zymomonas mobilis is unique among bacteria in its ability to produce high levels of ethanol (EtOH) during fermentation. Elevated EtOH concentration, like elevated temperature, is a microbial stress and a universal inducer of stress proteins. For Z. mobilis, exposure to high levels of EtOH represents a natural stress. By using a simple strategy which combines the genetic tools of Escherichia coli and Bacillus subtilis, we have cloned genes encoding two of the most abundant stress proteins in Z. mobilis, GroES and GroEL. Both genes were expressed at high levels in E. coli. Despite the unique environment (EtOH concentrations of above 10%) in which the translated products from these genes function in Z. mobilis, the amino-acid sequences encoded were remarkably similar to their homologues from bacteria which are not known to accumulate EtOH. Two small regions were observed, however, which appear more similar to Saccharomyces cerevisiae hsp60 (groEL homologue) than to E. coli or the concensus. These may be related to EtOH tolerance.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
148
pubmed:geneSymbol
groEL, groES
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
51-7
pubmed:dateRevised
2010-1-8
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Cloning, sequencing and expression of stress genes from the ethanol-producing bacterium Zymomonas mobilis: the groESL operon.
pubmed:affiliation
Department of Microbiology and Cell Science, University of Florida, Gainesville 32611.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't