Linked Life Data

7926768

Source:http://linkedlifedata.com/resource/pubmed/id/7926768

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1994-11-18
pubmed:databankReference
pubmed:abstractText
This report describes the identification, cloning, and molecular analysis of UME6 (CAR80/CARGRI), a key transcriptional regulator of early meiotic gene expression. Loss of UME6 function results in the accumulation of fully derepressed levels (70- to 100-fold increase above basal level) of early meiotic transcripts during vegetative growth. In contrast, mutations in five previously identified UME loci (UME1 to UME5), result in low to moderate derepression (2- to 10-fold increase) of early meiotic genes. The behavior of insertion and deletion alleles indicates that UME6 is dispensable for mitotic division but is required for meiosis and spore germination. Despite the high level of meiotic gene expression during vegetative growth, the generation times of ume6 mutant haploid and diploid cells are only slightly reduced. However, both ascus formation and spore viability are affected more severely. The UME6 gene encodes a 91-kD protein that contains a C6 zinc cluster motif similar to the DNA-binding domain of GAL4. The integrity of this domain is required for UME6 function. It has been reported recently that a mutation in CAR80 fails to complement an insertion allele of UME6. CAR80 is a gene required for nitrogen repression of the arginine catabolic enzymes. Here, through sequence analysis, we demonstrate that UME6 and CAR80 are identical. Analyses of UME6 mRNA during both nitrogen starvation and meiotic development indicate that its transcription is constitutive, suggesting that regulation of UME6 activity occurs at a post-transcriptional level.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
8
pubmed:geneSymbol
UME6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
796-810
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:7926768-Amino Acid Sequence, pubmed-meshheading:7926768-Base Sequence, pubmed-meshheading:7926768-Chromosome Mapping, pubmed-meshheading:7926768-Cloning, Molecular, pubmed-meshheading:7926768-DNA-Binding Proteins, pubmed-meshheading:7926768-Gene Expression Regulation, Fungal, pubmed-meshheading:7926768-Genes, Fungal, pubmed-meshheading:7926768-Genes, Regulator, pubmed-meshheading:7926768-Meiosis, pubmed-meshheading:7926768-Mitosis, pubmed-meshheading:7926768-Molecular Sequence Data, pubmed-meshheading:7926768-Mutation, pubmed-meshheading:7926768-Nitrogen, pubmed-meshheading:7926768-Polydeoxyribonucleotides, pubmed-meshheading:7926768-RNA, Fungal, pubmed-meshheading:7926768-Recombinant Fusion Proteins, pubmed-meshheading:7926768-Repressor Proteins, pubmed-meshheading:7926768-Saccharomyces cerevisiae, pubmed-meshheading:7926768-Saccharomyces cerevisiae Proteins, pubmed-meshheading:7926768-Sequence Analysis, DNA, pubmed-meshheading:7926768-Spores, Fungal, pubmed-meshheading:7926768-Transcription Factors, pubmed-meshheading:7926768-Zinc Fingers
pubmed:year
1994
pubmed:articleTitle
UME6 is a key regulator of nitrogen repression and meiotic development.
pubmed:affiliation
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't