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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1994-11-10
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pubmed:databankReference | |
pubmed:abstractText |
MbIRK3, mbGIRK2 and mbGIRK3 K+ channels cDNAs have been cloned from adult mouse brain. These cDNAs encode polypeptides of 445, 414 and 376 amino acids, respectively, which display the hallmarks of inward rectifier K+ channels, i.e. two hydrophobic membrane-spanning domains M1 and M2 and a pore-forming domain H5. MbIRK3 shows around 65% amino acid identity with IRK1 and rbIRK2 and only 50% with ROMK1 and GIRK1. On the other hand, mbGIRK2 and mbGIRK3 are more similar to GIRK1 (60%) than to ROMK1 and IRK1 (50%). Northern blot analysis reveals that these three novel clones are mainly expressed in the brain. Xenopus oocytes injected with mbIRK3 and mbGIRK2 cRNAs display inward rectifier K(+)-selective currents very similar to IRK1 and GIRK1, respectively. As expected from the sequence homology, mbGIRK2 cRNA directs the expression of G-protein coupled inward rectifier K+ channels which has been observed through their functional coupling with co-expressed delta-opioid receptors. These results provide the first evidence that the GIRK family, as the IRK family, is composed of multiple genes with members specifically expressed in the nervous system.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/G Protein-Coupled...,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Inwardly...,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-5793
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
353
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
37-42
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7926018-Amino Acid Sequence,
pubmed-meshheading:7926018-Animals,
pubmed-meshheading:7926018-Base Sequence,
pubmed-meshheading:7926018-Brain,
pubmed-meshheading:7926018-Cloning, Molecular,
pubmed-meshheading:7926018-DNA, Complementary,
pubmed-meshheading:7926018-G Protein-Coupled Inwardly-Rectifying Potassium Channels,
pubmed-meshheading:7926018-GTP-Binding Proteins,
pubmed-meshheading:7926018-Mice,
pubmed-meshheading:7926018-Molecular Sequence Data,
pubmed-meshheading:7926018-Potassium Channels,
pubmed-meshheading:7926018-Potassium Channels, Inwardly Rectifying,
pubmed-meshheading:7926018-RNA, Messenger,
pubmed-meshheading:7926018-Sequence Homology, Amino Acid,
pubmed-meshheading:7926018-Tissue Distribution,
pubmed-meshheading:7926018-Xenopus
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pubmed:year |
1994
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pubmed:articleTitle |
Cloning provides evidence for a family of inward rectifier and G-protein coupled K+ channels in the brain.
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pubmed:affiliation |
Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, Valbonne, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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