Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-11-4
|
| pubmed:abstractText |
Mitotically active Schwann cells isolated from adult rat sciatic nerve segments were cultivated, using a bivariate BrdU/DNA flow cytometry analysis, to test the effect of b-FGF (10 ng/ml), alpha-MSH (10 ng/ml), NGF (10 ng/ml), PDGF-AB (10 ng/ml), and TGF-beta 1 (10 ng/ml) on the cell cycle. Compared to control or cholera toxin-treated cultures, no significant differences (P < 0.05; Newmann-Keuls test) were observed in the proportion of G0G1 cells, S cells, G2M cells, and in the LI when alpha-MSH, NGF, PDGF-AB, or TGF-beta 1 were present. The S phase duration varied from 6.16 +/- 0.24 to 7.86 +/- 2.6 h, and the deduced potential doubling time was estimated at between 46.70 +/- 7.09 and 56.05 +/- 7.55 h. In contrast, when b-FGF was added to the culture, the cell cycle was significantly modified, and the proportion of G0G1 cells decreased from 68-77% to 59-64%, while the proportion of S cells increased from 14-16% to 24.0-26.4%. Although S phase duration was not significantly changed (6.02 +/- 0.36 h), the 1.7- to 2.8-fold LI increase reduced the potential doubling time to 25.99 +/- 6.13 h. We conclude from these results that only b-FGF-induced adult rat Schwann cells dramatically reenter in cell cycle and that this growth factor may be an axonally derived signal-promoting mitogenesis after nerve injury.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cholera Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Fibroblast Growth Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-MSH
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0014-4827
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
214
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
543-50
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7925648-Age Factors,
pubmed-meshheading:7925648-Animals,
pubmed-meshheading:7925648-Cell Cycle,
pubmed-meshheading:7925648-Cells, Cultured,
pubmed-meshheading:7925648-Cholera Toxin,
pubmed-meshheading:7925648-Culture Techniques,
pubmed-meshheading:7925648-Fibroblast Growth Factor 2,
pubmed-meshheading:7925648-Flow Cytometry,
pubmed-meshheading:7925648-Growth Substances,
pubmed-meshheading:7925648-Immunohistochemistry,
pubmed-meshheading:7925648-Microscopy, Phase-Contrast,
pubmed-meshheading:7925648-Nerve Growth Factors,
pubmed-meshheading:7925648-Platelet-Derived Growth Factor,
pubmed-meshheading:7925648-Rats,
pubmed-meshheading:7925648-Rats, Wistar,
pubmed-meshheading:7925648-Schwann Cells,
pubmed-meshheading:7925648-Sciatic Nerve,
pubmed-meshheading:7925648-Transforming Growth Factor beta,
pubmed-meshheading:7925648-alpha-MSH
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Establishment of adult rat Schwann cell cultures: effect of b-FGF, alpha-MSH, NGF, PDGF, and TGF-beta on cell cycle.
|
| pubmed:affiliation |
Pathology Laboratory, Charles Nicolle Hospital, Rouen, France.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|