Linked Life Data

7925613

Source:http://linkedlifedata.com/resource/pubmed/id/7925613

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-11-10
pubmed:abstractText
The possible mechanisms of antiproliferative effect of baicalein were studied in human T-lymphoid leukemia cells (CEM cells) and compared with those of esculetin. Baicalein, esculetin and related compounds, baicalein, wogonin, esculin and scoparone, inhibited CEM cell proliferation. Baicalein exhibited the greatest antiproliferative activity with an IC50 of 4.7 +/- 0.5 microM and the maximal suppression of 91.5 +/- 1.4% in CEM cells. The protein tyrosine kinase activity in the CEM cells was significantly reduced by baicalein (10(-6)-10(-4) M) and esculetin (10(-4) M). Baicalein exhibited a greater inhibitory activity on the protein tyrosine kinase than did esculetin (74.1 +/- 3.3% vs. 64.6 +/- 2.8% inhibition at 10(-4) M). On the other hand, the protein kinase C activity stimulated by phorbol-12-myristate 13-acetate was reduced by directly incubating with baicalein (10(-6)-10(-4) M) and esculetin (10(-4) M). However, the inhibitory activities on protein kinase C did not show a dose-dependency. The reverse transcription-polymerase chain reaction analysis of platelet-derived growth factor-A (PDGF-A) and transforming growth factor-beta 1 (TGF-beta 1) messenger RNA levels demonstrates that baicalein and esculetin reduced the PDGF-A mRNA level, but less affected the TGF-beta 1 mRNA. Baicalein exhibited the greater reduction on the expression of PDGF-A mRNA than did esculetin. It is suggested that baicalein and esculetin may affect cell proliferation by direct inhibition of growth-related signal, protein tyrosine kinase, as well as reduction of mRNA expression of growth factor, platelet-derived growth factor.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm, http://linkedlifedata.com/resource/pubmed/chemical/Flavanones, http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids, http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances, http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor, http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta, http://linkedlifedata.com/resource/pubmed/chemical/Umbelliferones, http://linkedlifedata.com/resource/pubmed/chemical/baicalein, http://linkedlifedata.com/resource/pubmed/chemical/esculetin
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0014-2999
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
268
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Effects of baicalein and esculetin on transduction signals and growth factors expression in T-lymphoid leukemia cells.
pubmed:affiliation
Department of Pharmacology, College of Medicine, National Taiwan University, Taipei, ROC.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't