Linked Life Data

7925493

Source:http://linkedlifedata.com/resource/pubmed/id/7925493

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-11-10
pubmed:abstractText
We have investigated the autophagocytic process of excess peroxisomes and mitochondria induced by di-(2-ethylhexyl)phthalate (DEHP) treatment using immunocytochemical techniques. Rat liver peroxisomes were induced by 2 weeks treatment with DEHP. The animals were then injected with leupeptin (2 mg/100 g body weight), and their livers were fixed by perfusion at various intervals. The liver tissues were embedded in LR White or Epon. Semithin sections of the Epon-embedded tissue were stained for cathepsin D, B, and H, and lysosomal glycoprotein (LGP107) by the immunoenzyme technique after removal of epoxy resin. Thin sections of LR White-embedded tissue were stained for the same antigens by the immunogold technique. Some liver specimens were processed to ultracryotomy, and frozen-thawed thin sections were immunostained for carboxylesterase E1 and alpha-glucosidase II, endoplasmic reticulum (ER) markers. Twenty minutes after leupeptin injection, many peroxisomes and mitochondria were surrounded by a double-layered membrane (isolation membrane) continuous with the ER. These membranes were positive for carboxylesterase E1 and alpha-glucosidase, but not for LGP107 as well as cathepsins. Forty to 60 minutes after leupeptin injection many autophagic vacuoles showing various developing stages appeared and accumulated. The early autophagic vacuoles were surrounded by a double-layered membrane, whereas the late autophagic vacuoles had a single limiting membrane. The former was negative for cathepsins as well as LGP107, but positive for carboxylesterase E1 and alpha-glucosidase II. The results suggest strongly that the isolation membrane is derived from the ER membrane and converted later into the lysosomal membrane and support our previous morphological observations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0171-9335
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
372-83
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Formation of autophagosomes during degradation of excess peroxisomes induced by di-(2-ethylhexyl)phthalate treatment. II. Immunocytochemical analysis of early and late autophagosomes.
pubmed:affiliation
Department of Anatomy, Yamanashi Medical School, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't