Linked Life Data

7921263

Source:http://linkedlifedata.com/resource/pubmed/id/7921263

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-11-21
pubmed:databankReference
pubmed:abstractText
Major immunogenic polypeptides (21, 32, 40, 46, 58, 85 and 160 kDa) of Cowdria ruminantium were identified by immunoprecipitation and immunoblotting. A pUC13 library of C. ruminantium genomic DNA was screened with hyperimmune sheep serum to identify Escherichia coli colonies which expressed genes encoding these immunogenic proteins. A recombinant E. coli colony, F5.2, was identified containing plasmid insert DNA of 2773 bp. The cloned DNA insert contained two long open reading frames (ORFs) of 627 bp (complete) and 831 bp (incomplete), both potentially encoding proteins containing an N-terminal signal peptide. Deletion experiments suggested that the hyperimmune sheep serum recognized a protein that was encoded by the 627 bp ORF. The 627 bp ORF was amplified by polymerase chain reaction (PCR), subcloned and expressed to a high level in E. coli. A sheep antiserum made to the expressed recombinant fusion protein recognized a 21 kDa protein of all strains of C. ruminantium tested, confirming that the 627 bp ORF encodes a native 21 kDa protein in C. ruminantium. Similarly, the recombinant protein was recognized by all sera tested from heartwater-infected animals. The antigenic conservation of the 21 kDa protein and its immunogenic nature are reasons for further testing of this recombinant protein in subunit diagnostic tests.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
140 ( Pt 8)
pubmed:geneSymbol
ptsH, ptsI, tna, tnaA, tnaB
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2135-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7921263-Amino Acid Sequence, pubmed-meshheading:7921263-Animals, pubmed-meshheading:7921263-Antigens, Bacterial, pubmed-meshheading:7921263-Bacterial Outer Membrane Proteins, pubmed-meshheading:7921263-Bacterial Proteins, pubmed-meshheading:7921263-Base Sequence, pubmed-meshheading:7921263-Cloning, Molecular, pubmed-meshheading:7921263-DNA, Bacterial, pubmed-meshheading:7921263-Ehrlichia ruminantium, pubmed-meshheading:7921263-Genes, Bacterial, pubmed-meshheading:7921263-Heartwater Disease, pubmed-meshheading:7921263-Molecular Sequence Data, pubmed-meshheading:7921263-Recombinant Proteins, pubmed-meshheading:7921263-Sequence Alignment, pubmed-meshheading:7921263-Sequence Homology, Amino Acid, pubmed-meshheading:7921263-Species Specificity, pubmed-meshheading:7921263-Zimbabwe
pubmed:year
1994
pubmed:articleTitle
Molecular cloning of a gene encoding the immunogenic 21 kDa protein of Cowdria ruminantium.
pubmed:affiliation
Department of Infectious Diseases, College of Veterinary Medicine, University of Florida, Gainesville 32611-0880.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, Non-P.H.S.