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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1994-11-17
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pubmed:abstractText |
Anthopleurin-A (AP-A) is a member of a family of sea anemone-derived polypeptides that interact with sodium channels in a voltage-dependent manner, producing a positive inotropic effect on the mammalian heart. There has been considerable interest in this molecule as a lead compound for the development of novel therapeutic agents. Earlier attempts to define the 3-dimensional structure of AP-A were complicated by the fact that it was found to exist in 2 conformations in solution. Using 1H- and 13C-NMR spectroscopy, we have now shown that this conformational heterogeneity arises from cis-trans isomerization about the Gly 40-Pro 41 peptide bond and that in the major form of the protein this peptide bond adopts a cis conformation. Furthermore, the increased sensitivity afforded by higher-field NMR has allowed identification of additional minor conformations of AP-A, the origin of which is presently unknown. We believe there will be many more examples of the detection by high-field NMR of previously unobserved minor conformations of proteins in solution.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7920260-1686683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7920260-1968006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7920260-2905176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7920260-3627269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7920260-4076190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7920260-6147271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7920260-6307305,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7920260-712617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7920260-7682109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7920260-8381876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7920260-94413
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0961-8368
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
3
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1121-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1994
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pubmed:articleTitle |
Multiple conformations of the sea anemone polypeptide anthopleurin-A in solution.
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pubmed:affiliation |
NMR Laboratory, Biomolecular Research Institute, Parkville, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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