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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1994-11-15
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pubmed:abstractText |
In this paper we describe a complete deletional analysis of hypersensitive site three (HS3) of the human beta-globin Locus Control Region (LCR). The previously defined core fragment consists of 6 footprinted regions, with multiple binding sites for the erythroid-specific factor GATA-1 and G-rich motifs that can interact with ubiquitous factors such as Sp1 and TEF-2. We show in this paper that the 5' half of this fragment is the most important for activity in murine erythroleukemia (MEL) cells. A fragment containing footprints 1-4 can stimulate transcription of a linked human beta-globin gene to levels of about 40% of that obtained with footprints 1-6. Constructs containing either footprints 1-3 or 3-6 cannot be distinguished from the beta-globin gene alone. We further show that binding sites for the erythroid-specific factor NF-E2 can co-operatively interact with parts of the HS3 core fragment, and that HS3 requires elements upstream from -103 in the human beta-globin promoter for full activity. The importance of these results is discussed in the context of the regulation of the genes in the human beta-globin cluster.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Erythroid-Specific DNA-Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/GATA1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Gata1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Globins,
http://linkedlifedata.com/resource/pubmed/chemical/NF-E2 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/NF-E2 Transcription Factor, p45...,
http://linkedlifedata.com/resource/pubmed/chemical/NFE2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nfe2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-3002
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
1219
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
351-60
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7918632-Animals,
pubmed-meshheading:7918632-Base Sequence,
pubmed-meshheading:7918632-Binding Sites,
pubmed-meshheading:7918632-DNA Primers,
pubmed-meshheading:7918632-DNA-Binding Proteins,
pubmed-meshheading:7918632-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:7918632-GATA1 Transcription Factor,
pubmed-meshheading:7918632-Gene Expression Regulation,
pubmed-meshheading:7918632-Globins,
pubmed-meshheading:7918632-Humans,
pubmed-meshheading:7918632-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:7918632-Mice,
pubmed-meshheading:7918632-Molecular Sequence Data,
pubmed-meshheading:7918632-NF-E2 Transcription Factor,
pubmed-meshheading:7918632-NF-E2 Transcription Factor, p45 Subunit,
pubmed-meshheading:7918632-Promoter Regions, Genetic,
pubmed-meshheading:7918632-Sequence Deletion,
pubmed-meshheading:7918632-Transcription, Genetic,
pubmed-meshheading:7918632-Transcription Factors,
pubmed-meshheading:7918632-Transfection,
pubmed-meshheading:7918632-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Transcriptional activation by hypersensitive site three of the human beta-globin locus control region in murine erythroleukemia cells.
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pubmed:affiliation |
National Institute for Medical Research, London, UK.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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