| pubmed-article:7918619 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7918619 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7918619 | lifeskim:mentions | umls-concept:C0040162 | lld:lifeskim |
| pubmed-article:7918619 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:7918619 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:7918619 | lifeskim:mentions | umls-concept:C0009017 | lld:lifeskim |
| pubmed-article:7918619 | lifeskim:mentions | umls-concept:C1335671 | lld:lifeskim |
| pubmed-article:7918619 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:7918619 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:7918619 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:7918619 | pubmed:dateCreated | 1994-11-15 | lld:pubmed |
| pubmed-article:7918619 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7918619 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7918619 | pubmed:abstractText | In this study, we isolated genomic DNA fragments coding for the human thyrotropin-releasing hormone (TRH) receptor. Analysis of the nucleotide sequence revealed that the human TRH receptor gene had an exon-intron structure comprising at least two exons. A polypeptide encoded by the gene consisted of 398 amino acid residues with putative seven transmembrane domains. It showed high homology as a whole amino acid sequence with the rat and mouse TRH receptors except for considerable variation in the C-terminal region. Chromosomal mapping study indicated that the human TRH receptor gene was assigned to chromosome 8. Chinese hamster ovary (CHO) cells transfected with a DNA fragment containing the coding regions of the human TRH receptor bound with [3H]TRH. This binding was inhibited by adding unlabeled TRH in a dose-dependent fashion. Scatchard analysis indicated that the transfected CHO cells expressed a single class of high affinity binding sites at a dissociation constant (Kd) of approximately 1 nM. These results demonstrated that the isolated gene encoded a specific TRH receptor with high affinity. | lld:pubmed |
| pubmed-article:7918619 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7918619 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7918619 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7918619 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7918619 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7918619 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7918619 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7918619 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7918619 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7918619 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7918619 | pubmed:issn | 0006-3002 | lld:pubmed |
| pubmed-article:7918619 | pubmed:author | pubmed-author:NagaiYY | lld:pubmed |
| pubmed-article:7918619 | pubmed:author | pubmed-author:TanakaHH | lld:pubmed |
| pubmed-article:7918619 | pubmed:author | pubmed-author:NEELH BHB | lld:pubmed |
| pubmed-article:7918619 | pubmed:author | pubmed-author:HinumaSS | lld:pubmed |
| pubmed-article:7918619 | pubmed:author | pubmed-author:MALUFN S RNS | lld:pubmed |
| pubmed-article:7918619 | pubmed:author | pubmed-author:HosoyaMM | lld:pubmed |
| pubmed-article:7918619 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7918619 | pubmed:day | 18 | lld:pubmed |
| pubmed-article:7918619 | pubmed:volume | 1219 | lld:pubmed |
| pubmed-article:7918619 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7918619 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7918619 | pubmed:pagination | 251-9 | lld:pubmed |
| pubmed-article:7918619 | pubmed:dateRevised | 2005-11-17 | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:meshHeading | pubmed-meshheading:7918619-... | lld:pubmed |
| pubmed-article:7918619 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7918619 | pubmed:articleTitle | Molecular cloning and functional expression of a human thyrotropin-releasing hormone (TRH) receptor gene. | lld:pubmed |
| pubmed-article:7918619 | pubmed:affiliation | Discovery Research Laboratories I, Takeda Chemical Industries, Ltd., Ibaraki, Japan. | lld:pubmed |
| pubmed-article:7918619 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:7201 | entrezgene:pubmed | pubmed-article:7918619 | lld:entrezgene |
