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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1993-5-28
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D12906,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D12907,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03653,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L03654,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L07631,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L07632,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M97164,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S59893,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X54209,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X67963
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pubmed:abstractText |
A cDNA library in lambda Charon BS(-) from 11-week-old human fetal brain (FB) was screened using a human liver ferritin (FTH)-encoding cDNA as a probe. The complete sequence of the positive clone, cFB1, showed that the coding region and a part of the 5' and 3' untranslated regions (UTR) are identical to the corresponding published sequence of the liver cDNA. However, a particularly noteworthy difference is the presence of 279 bp of additional sequence in the FB 3'-UTR. Northern blot analysis of FB poly(A)+RNA showed it to be a part of the FTH transcript. Comparison of the 279-bp sequence with the GenBank and EMBL databases showed it to be 94.1, 62.5, and 58.9% similar to segments from human, mouse and rat FTH genomic sequences, respectively. However, in all these cases, only a part of this 279-bp sequence has been found in the nontranscribed region. We therefore conclude that in FB, the 279-bp sequence is a part of the mature FTH mRNA. Sequence analysis also suggests a differential poly(A) site selection in the production of FTH mRNA in FB and liver.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Apr
|
pubmed:issn |
0378-1119
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
30
|
pubmed:volume |
126
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
275-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7916709-Amino Acid Sequence,
pubmed-meshheading:7916709-Base Sequence,
pubmed-meshheading:7916709-Brain,
pubmed-meshheading:7916709-Cloning, Molecular,
pubmed-meshheading:7916709-DNA,
pubmed-meshheading:7916709-Ferritins,
pubmed-meshheading:7916709-Fetus,
pubmed-meshheading:7916709-Humans,
pubmed-meshheading:7916709-Molecular Sequence Data,
pubmed-meshheading:7916709-Sequence Homology, Nucleic Acid,
pubmed-meshheading:7916709-Transcription, Genetic
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pubmed:year |
1993
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pubmed:articleTitle |
Sequence of a cDNA encoding the ferritin H-chain from an 11-week-old human fetal brain.
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pubmed:affiliation |
Department of Biochemistry, University of Tennessee, Knoxville 37996-0840.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|