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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1993-10-15
|
| pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L09682,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L09683,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X71339,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X71340,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X72968,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X73358,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X73359,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X73360,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X73361,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X73887
|
| pubmed:abstractText |
We have isolated and analysed the 5' flanking region of the rat acetylcholine receptor (AChR) beta subunit gene and determined regulatory elements that confer muscle specificity. Deletion mapping revealed a minimal TATA-box-less promoter region containing an initiator motif. An 85-bp fragment has been shown to promote high muscle-specific expression of a chloramphenicol acetyltransferase (CAT) reporter construct upon transfection in primary muscle cells. This sequence can be functionally dissected in a basal muscle-specific promoter element carrying a M-CAT box that is flanked at the 5' end by an enhancer element with two binding sites for myogenic factors. Point mutations in the M-CAT box cause the loss of transcriptional activity of the basal promoter fragment. The enhancer activity depends on the presence of both E boxes that cooperate in a synergistic fashion. We therefore conclude that the control of muscle-specific and developmental expression of the rat AChR beta subunit gene requires both regulatory elements, the M-CAT box and two adjacent E boxes, located in close proximity to each other. Cotransfection experiments in NIH3T3 cells demonstrate that the rat AChR beta subunit gene can be transactivated by myogenic factors displaying a preference for myogenin, as well as MRF4 and myf5 compared to a clearly weaker responsiveness to MyoD1.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0014-2956
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
216
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
395-404
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:7916688-3T3 Cells,
pubmed-meshheading:7916688-Animals,
pubmed-meshheading:7916688-Base Sequence,
pubmed-meshheading:7916688-Cells, Cultured,
pubmed-meshheading:7916688-DNA,
pubmed-meshheading:7916688-Gene Expression Regulation,
pubmed-meshheading:7916688-HeLa Cells,
pubmed-meshheading:7916688-Humans,
pubmed-meshheading:7916688-Mice,
pubmed-meshheading:7916688-Molecular Sequence Data,
pubmed-meshheading:7916688-Muscle Proteins,
pubmed-meshheading:7916688-Muscles,
pubmed-meshheading:7916688-Point Mutation,
pubmed-meshheading:7916688-Promoter Regions, Genetic,
pubmed-meshheading:7916688-Rats,
pubmed-meshheading:7916688-Receptors, Nicotinic,
pubmed-meshheading:7916688-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7916688-Sequence Deletion,
pubmed-meshheading:7916688-Transcriptional Activation
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Two adjacent E box elements and a M-CAT box are involved in the muscle-specific regulation of the rat acetylcholine receptor beta subunit gene.
|
| pubmed:affiliation |
Max-Planck-Institut für medizinische Forschung, Abteilung Zellphysiologie, Heidelberg, Germany.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|