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rdf:type |
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lifeskim:mentions |
umls-concept:C0009015,
umls-concept:C0016976,
umls-concept:C0017262,
umls-concept:C0030274,
umls-concept:C0085979,
umls-concept:C0185117,
umls-concept:C0205280,
umls-concept:C0250571,
umls-concept:C0449560,
umls-concept:C0597357,
umls-concept:C2911684
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pubmed:issue |
6 Pt 1
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pubmed:dateCreated |
1994-2-8
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pubmed:databankReference |
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pubmed:abstractText |
Cholecystokinin (CCK) receptors mediate pancreatic acinar secretion and gallbladder contraction. Pharmacological and functional studies in pancreas and gallbladder demonstrate a CCK-A receptor subtype in both tissues. However, some pharmacological studies and affinity cross-linking studies of CCK receptors on pancreatic acini and gallbladder suggest that these two tissues possess two different subtypes of the CCK-A receptor. We cloned these receptors in guinea pig using a cDNA clone of the CCK-A receptor from rat pancreas. The guinea pig gallbladder CCK-A receptor was cloned by hybridization screening of a gallbladder cDNA library using a cDNA probe from the rat CCK-A receptor coding region. The guinea pig pancreas CCK-A receptor cDNA was cloned via the polymerase chain reaction using primers corresponding to the guinea pig gallbladder CCK-A receptor 5'- and 3'-noncoding regions. CCK-A receptor clones from guinea pig pancreas and gallbladder had identical nucleotide sequences, which were 80% homologous to the rat CCK-A receptor cDNA sequence. The deduced amino acid sequence from guinea pig CCK-A receptors was 89% homologous to the rat CCK-A receptor sequence. Dose-inhibition binding studies of transiently expressed receptors by CCK agonists and antagonists exhibited a CCK-A receptor pharmacologically similar to the rat CCK-A receptor. These studies indicate that the CCK-A receptors in guinea pig pancreas and gallbladder are identical and do not support previous proposals that they may represent different receptor subtypes.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bolton Hunter-cholecystokinin...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cholecystokinin,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sincalide,
http://linkedlifedata.com/resource/pubmed/chemical/Succinimides
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
265
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
G1116-21
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7916580-Amino Acid Sequence,
pubmed-meshheading:7916580-Animals,
pubmed-meshheading:7916580-Base Sequence,
pubmed-meshheading:7916580-Blotting, Northern,
pubmed-meshheading:7916580-Cell Line,
pubmed-meshheading:7916580-Cloning, Molecular,
pubmed-meshheading:7916580-DNA, Complementary,
pubmed-meshheading:7916580-DNA Primers,
pubmed-meshheading:7916580-Gallbladder,
pubmed-meshheading:7916580-Gene Expression,
pubmed-meshheading:7916580-Gene Library,
pubmed-meshheading:7916580-Guinea Pigs,
pubmed-meshheading:7916580-Liver,
pubmed-meshheading:7916580-Male,
pubmed-meshheading:7916580-Molecular Sequence Data,
pubmed-meshheading:7916580-Muscles,
pubmed-meshheading:7916580-Organ Specificity,
pubmed-meshheading:7916580-Pancreas,
pubmed-meshheading:7916580-Polymerase Chain Reaction,
pubmed-meshheading:7916580-RNA, Messenger,
pubmed-meshheading:7916580-Rats,
pubmed-meshheading:7916580-Receptor, Cholecystokinin A,
pubmed-meshheading:7916580-Receptors, Cholecystokinin,
pubmed-meshheading:7916580-Recombinant Proteins,
pubmed-meshheading:7916580-Sequence Homology, Amino Acid,
pubmed-meshheading:7916580-Sincalide,
pubmed-meshheading:7916580-Succinimides,
pubmed-meshheading:7916580-Transfection
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pubmed:year |
1993
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pubmed:articleTitle |
Guinea pig gallbladder and pancreas possess identical CCK-A receptor subtypes: receptor cloning and expression.
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pubmed:affiliation |
Digestive Diseases Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
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pubmed:publicationType |
Journal Article,
Comparative Study
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