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| pubmed-article:7916512 | pubmed:abstractText | The primary targets for infection of HIV-1 are CD4+ T cells and monocyte/macrophage cells. We investigated the genotype heterogeneity of HIV-1 in distinct blood compartments, CD4+ T cells, monocytes, and plasma. Nucleotide sequences of proviral DNA from CD4+ T cells, monocyte cells, and cell-free virion RNA from plasma were determined in three HIV-1 infected individuals. We found that the heterogeneity in proviral DNA from CD4+ T cells was much higher than that found in monocyte cells or in virion RNA from plasma. These data showed that different swarms of HIV-1 exist in individually infected cells and plasma. Furthermore, predicted V3 amino acid sequences in the infected monocytes were very similar to the reported sequence of prototypic macrophage-tropic HIV-1 isolates. Our results show that macrophage-tropic virus is quite homogeneous in monocytes/macrophages and has a common V3 structure in contrast to T-cell-tropic virus in vivo. | lld:pubmed |
| pubmed-article:7916512 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:7916512 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7916512 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7916512 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7916512 | pubmed:issn | 0042-6822 | lld:pubmed |
| pubmed-article:7916512 | pubmed:author | pubmed-author:YamashitaAA | lld:pubmed |
| pubmed-article:7916512 | pubmed:author | pubmed-author:YamamotoNN | lld:pubmed |
| pubmed-article:7916512 | pubmed:author | pubmed-author:MatsudaJJ | lld:pubmed |
| pubmed-article:7916512 | pubmed:author | pubmed-author:KoyanagiYY | lld:pubmed |
| pubmed-article:7916512 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7916512 | pubmed:volume | 204 | lld:pubmed |
| pubmed-article:7916512 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7916512 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7916512 | pubmed:pagination | 170-9 | lld:pubmed |
| pubmed-article:7916512 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:7916512 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7916512 | pubmed:articleTitle | Cell type-specific heterogeneity of the HIV-1 V3 loop in infected individuals: selection of virus in macrophages and plasma. | lld:pubmed |
| pubmed-article:7916512 | pubmed:affiliation | Department of Microbiology, Tokyo Medical and Dental University, Japan. | lld:pubmed |
| pubmed-article:7916512 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7916512 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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