Linked Life Data

7916313

Source:http://linkedlifedata.com/resource/pubmed/id/7916313

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-10-14
pubmed:abstractText
Two plasmid expression vectors for Bacillus subtilis have been constructed that direct the synthesis of fusion proteins containing a stretch of six histidine residues (His6) at either the N or C terminus. The His6 tag allows the rapid enrichment of proteins by metal chelate affinity chromatography in a denatured or native state. As an example of the general utility of one of these expression vectors, we produced the GroES protein encoded by the groESL operon of B. stearothermophilus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
147
pubmed:geneSymbol
groES
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
91-4
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Construction of His6-tagging vectors allowing single-step purification of GroES and other polypeptides produced in Bacillus subtilis.
pubmed:affiliation
Lehrstuhl für Genetik, Universität Bayreuth, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't