Linked Life Data

7915950

Source:http://linkedlifedata.com/resource/pubmed/id/7915950

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-10-10
pubmed:abstractText
Keratinocytes are activated to express MHC class II and ICAM-1 molecules during cutaneous inflammatory reactions. It is controversial how the interaction between these 'nonprofessional' antigen presenting cells (APC) and infiltrating T cells affects the local inflammatory response. To address this issue we analyzed whether IFN gamma-treated cultured human keratinocytes would activate established Th cell clones in vitro. Three allo DR specific T cell clones were induced to proliferate in a HLA-DR and LFA-1/ICAM-1 dependent fashion upon coculture with intact layers of IFN gamma stimulated keratinocytes. Likewise, keratinocytes also could activate two out of four minor histocompatibility (mH) antigen specific Th cell clones obtained from peripheral blood leukocytes (PBL) of graft versus host disease patients. The T cell activating potential of MHC class II+ keratinocytes was shown to be relatively low compared to specialized APC as PMNC and EBV-BLCL. Most strikingly, measurable allo MHC and mH antigen specific Th cell proliferation was only induced by using adherent keratinocytes at low cell densities, but not by keratinocytes in suspension. The results presented here indicate that in vitro conditions may crucially influence observations regarding the T cell activating potential of MHC class II expressing keratinocytes. Furthermore, our results indicate that, in addition to a tolerizing effect as suggested by previous reports, interaction of primed antigen specific T cells with activated keratinocytes may also result in enhancement of a cutaneous immune response in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0966-3274
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
52-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:7915950-Antigen-Presenting Cells, pubmed-meshheading:7915950-Cell Adhesion Molecules, pubmed-meshheading:7915950-Cell Count, pubmed-meshheading:7915950-Female, pubmed-meshheading:7915950-H-Y Antigen, pubmed-meshheading:7915950-HLA-DR Antigens, pubmed-meshheading:7915950-Humans, pubmed-meshheading:7915950-Immune Tolerance, pubmed-meshheading:7915950-Intercellular Adhesion Molecule-1, pubmed-meshheading:7915950-Interferon-gamma, pubmed-meshheading:7915950-Keratinocytes, pubmed-meshheading:7915950-Lymphocyte Activation, pubmed-meshheading:7915950-Lymphocyte Culture Test, Mixed, pubmed-meshheading:7915950-Male, pubmed-meshheading:7915950-Minor Histocompatibility Antigens, pubmed-meshheading:7915950-Recombinant Proteins, pubmed-meshheading:7915950-T-Lymphocytes, Helper-Inducer
pubmed:year
1993
pubmed:articleTitle
Human keratinocytes activate primed major and minor histocompatibility antigen specific Th cells in vitro.
pubmed:affiliation
Department of Immunohaematology, University Hospital, Leiden, The Netherlands.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't