Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-9-26
pubmed:abstractText
Multiple gamma-glutamyl transpeptidase (GGT) messenger RNAs (mRNAsII-IV) are expressed in a region-specific manner in the rat epididymis. In the present study, we examined the role(s) of plasma testosterone (T) and testicular factors in regulating the region-specific pattern and quantity of GGT mRNAs expressed along the epididymal duct. Northern blot and ribonuclease protection analyses showed that bilateral orchiectomy for 1, 5, and 15 days dramatically reduced the expression of GGT mRNAsII-IV in the initial segment. Expression of GGT mRNAII and mRNAIII was maintained in the initial segment of orchiectomized animals receiving T implants that maintain normal serum T concentrations, but GGT mRNAIV expression remained low relative to sham-operated control values. Unilateral efferent duct ligation decreased GGT mRNAIV expression only in the initial segment. Hence, expression of GGT mRNAIV in the initial segment was not maintained by circulating T and required a factor(s) of testicular origin. In caput epididymidis, expression of GGT mRNAII and mRNAIII declined after orchiectomy and was not completely restored to control values in orchiectomized animals by plasma T alone, but also required a testicular factor(s). In contrast to the initial segment, expression of GGT mRNAIV in the corpus and cauda epididymidis did not require T and/or a testicular factor(s), as expression of this transcript remained unchanged in these regions after 1, 5, and 15 days of orchiectomy, orchiectomy and T replacement, and after unilateral efferent duct ligation. In the cauda epididymidis, expression of GGT mRNAII required circulating androgens and was unaffected by unilateral efferent duct ligation, whereas GGT mRNAIII expression was repressed by T. These data demonstrate that circulating T and a factor(s) of testicular origin differentially regulate the expression of each GGT mRNA in a region-specific manner.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
135
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1146-56
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Expression of multiple gamma-glutamyl transpeptidase messenger ribonucleic acid transcripts in the adult rat epididymis is differentially regulated by androgens and testicular factors in a region-specific manner.
pubmed:affiliation
Department of Cell Biology, University of Virginia Health Sciences Center, Charlottesville 22908.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't