Linked Life Data

7915194

Source:http://linkedlifedata.com/resource/pubmed/id/7915194

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
1994-9-27
pubmed:abstractText
The mouse mdr gene family is composed of three members designated mdr1, mdr2, and mdr3. A full-length mdr2 complementary DNA clone has been introduced in an amplifiable eukaryotic expression vector (pEMC2b1) which directs amplification and overexpression of a bicistronic mdr2-dihydrofolate reductase mRNA after stepwise methotrexate selection of transfected mutant dihydrofolate reductase Chinese hamster ovary DUK cells. Independent cell clones expressing low to high amounts of mdr2 cellular mRNA and Mdr2 protein in their membrane fraction could be obtained by this selection procedure. Comparison of drug survival characteristics of cell clones expressing similar amounts of either Mdr1 or Mdr2 proteins revealed that Mdr1 but not Mdr2 could confer readily detectable levels of colchicine or vinblastine resistance. Labeling experiments using membrane-enriched fractions and a photoactivatable analogue of ATP showed that the Mdr2 protein was properly inserted in the membrane of transfected cells and could bind this ligand with an apparent affinity similar to that of Mdr1. However, labeling studies with the photoactivatable drug analogue iodoarylazidoprazosin showed considerably reduced binding of this ligand to Mdr2 as compared to Mdr1. Our findings demonstrate that Mdr2 cannot confer drug resistance and suggest that this inability is linked to reduced drug binding to the Mdr2 protein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
54
pubmed:geneSymbol
mdr2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4892-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
The inability of the mouse mdr2 gene to confer multidrug resistance is linked to reduced drug binding to the protein.
pubmed:affiliation
Department of Biochemistry, McGill University, Montreal, Quebec, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't