Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-9-21
pubmed:abstractText
Short-term exposure of isolated rat hepatocytes to short- and medium-chain fatty acids led to an activation of acetyl-CoA carboxylase as measured in digitonin-permeabilized hepatocytes. Up to a certain concentration, typical for each of the fatty acids used, fatty acid-dependent activation of acetyl-CoA carboxylase coincided with an increase in the rate of fatty acid synthesis in intact hepatocytes, as determined by the incorporation of 3H from 3H2O water into fatty acids. At higher concentrations loss of stimulation of fatty acid synthesis occurred, but not the enhancement of carboxylase activity. With the fatty acids tested (C8:0-C14:0), the peak in fatty acid synthesis coincided with a peak in the level of malonyl-CoA. The onset of the stimulation of carboxylase activity coincided with the start of the peak in both fatty acid synthesis and malonyl-CoA. The longer the chain length of the fatty acid added, the lower the concentration at which the rate of fatty acid synthesis and the level of malonyl-CoA reached a peak and carboxylase activity started to become elevated. In cell suspensions incubated with increasing concentrations of fatty acids, accumulation of lactate decreased progressively. The latter observation, in combination with the fact that the activity of acetyl-CoA carboxylase is not always related to the rate of fatty acid biosynthesis, suggests that under these conditions not the activity of the carboxylase but the flux through the glycolytic sequence determines, at least in part, the rate of fatty acid synthesis de novo.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-1134497, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-1536860, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-1680322, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-169739, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-177845, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-199159, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-2093139, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-2187945, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-2882781, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-31303, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-3196710, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-3401217, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-4413041, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-4433540, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-4643352, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-4653823, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-467808, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-4705268, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-4851629, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-5749773, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-5875764, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-6108268, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-6124242, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-6148077, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-6157353, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-6317044, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-6814231, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-7294812, http://linkedlifedata.com/resource/pubmed/commentcorrection/7915110-7378059
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
302 ( Pt 1)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
141-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Medium-chain fatty acids as short-term regulators of hepatic lipogenesis.
pubmed:affiliation
Laboratory of Veterinary Biochemistry, Utrecht, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't