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pubmed:otherAbstract |
PIP: The use of aqueous chlorhexidine (CHD) for vas irrigation and the clinical application of trypan blue uptake in the study of the viability of nonmotile sperms following vasectomy were evaluated. At 6 weeks postvasectomy, 28 percent of the patients irrigated with water and only 7 percent of those irrigated with CHD showed sperms, while 59 percent of the nonirrigated group showed sperms. In all cases, the sperms were immotile. In the trypan blue studies, 31 of 61 semen samples had sperm counts of less than 1 x 10 less than 6 greater than, 87 percent of which had an absolute uptake of 100 percent. In the remaining samples with higher sperm counts, 53 percent had a mean uptake of 100 percent. The results demonstrate the spermicidal effect of aqueous CHD. It is concluded that the trypan blue uptake test is the most objective for determining the motility of sperm, short of direct test tube fertilization of ova.
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