Linked Life Data

7913074

Source:http://linkedlifedata.com/resource/pubmed/id/7913074

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-8-8
pubmed:abstractText
1. The effect of gestagens, 5 alpha-hydroxyprogesterone (10(-6) x 10(-5) M), 5 beta-hydroxyprogesterone (10(-6)-3 x 10(-5) M), progesterone (6 x 10(-6)-10(-4) M), pregnanolone (10(-6)-10(-5) M), allopregnanolone (10(-6)-10(-4) M) and epipregnanolone (10(-6)-6 x 10(-5) M) on rat uterine contractions induced by KCl (60 mM), has been assayed. 2. All drugs assayed relaxed the tonic-contraction induced by KCl in a concentration-dependent way. The respectives IC50 were 31.3 +/- 4.1 x 10(-6) M (progesterone), 8.9 +/- 0.8 x 10(-6) M (5 alpha-hydroxyprogesterone), 3.8 +/- 0.3 x 10(-6) M (5 beta-hydroxyprogesterone), 3.1 +/- 0.1 x 10(-6) M (pregnanolone), 21.2 +/- 3.1 x 10(-6) M (allopregnanolone) and 6.3 +/- 1.3 x 10(-6) M (epipregnanolone). This relaxing effect was partially or totally counteracted by CaCl2 (1-10 mM) 3. Cycloheximide (10 micrograms/ml) significantly shifted to the right the effect of allopregnanolone but not the effect of the other drugs. Actinomycin D (5 micrograms/ml) did not modify the effect of allopregnanolone. 4. Our results suggest that the relaxing effect of gestagens in the rat uterus could be related to inhibition on calcium influx and mainly occur through non-genomic mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0306-3623
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
173-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Progesterone and pregnanolone derivatives relaxing effect on smooth muscle.
pubmed:affiliation
Departamento de Medicina, Universidad de Oviedo, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't