Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1994-8-10
|
| pubmed:abstractText |
The effect of the neurotoxin domoic acid (DOM), a structural analogue of kainic acid, on the release of [3H]gamma-aminobutyric acid (GABA) and on the [Ca2+]i was studied in cultured chick retina cells. DOM stimulated dose-dependently the release of [3H]GABA with an EC50 of 2.5 microM. In Ca(2+)-containing medium (1 mM), DOM (5 microM) increased the [Ca2+]i by about 190 nM and evoked the release of 11.8 +/- 1.3% of the intracellular [3H]GABA, while in the absence of extracellular Ca2+ DOM induced the release of only 7.9 +/- 1.4% of the accumulated [3H]GABA. The Ca(2+)-independent release of [3H]GABA was blocked by the non-competitive inhibitor of the GABA carrier 1-(2-(((diphenylmethylene)amino)oxy)ethyl)-1,2,5,6-tetrahydro-3-py ridine- carboxylic acid hydrochloride (NNC-711), but a component of Ca(2+)-dependent release remains. DOM evoked Ca(2+)-independent release of [3H]GABA was significantly depressed in the absence of external Na+ and completely blocked by the non-selective antagonist of the non-NMDA glutamate receptors, 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). Similarly, CNQX decreased the [Ca2+]i response to DOM, whereas L(+)-2-amino-3-phosphonopropionic acid (L-AP3), an antagonist of the metabotropic glutamate receptors, was without effect. MK-801 did not affect the release of [3H]GABA stimulated by DOM. Taken together our results indicate that DOM evokes both Ca(2+)-dependent and Ca(2+)-independent release of [3H]GABA, most likely by activating kainate receptors.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/6-Cyano-7-nitroquinoxaline-2,3-dione,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/GABA Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Kainic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/NNC 711,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Nicotinic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Nipecotic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Oximes,
http://linkedlifedata.com/resource/pubmed/chemical/Quinoxalines,
http://linkedlifedata.com/resource/pubmed/chemical/domoic acid,
http://linkedlifedata.com/resource/pubmed/chemical/gamma-Aminobutyric Acid
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0197-0186
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
24
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
267-74
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:7912974-6-Cyano-7-nitroquinoxaline-2,3-dione,
pubmed-meshheading:7912974-Animals,
pubmed-meshheading:7912974-Calcium,
pubmed-meshheading:7912974-Cells, Cultured,
pubmed-meshheading:7912974-Chick Embryo,
pubmed-meshheading:7912974-Excitatory Amino Acid Antagonists,
pubmed-meshheading:7912974-GABA Antagonists,
pubmed-meshheading:7912974-Kainic Acid,
pubmed-meshheading:7912974-Neurotoxins,
pubmed-meshheading:7912974-Nicotinic Acids,
pubmed-meshheading:7912974-Nipecotic Acids,
pubmed-meshheading:7912974-Oximes,
pubmed-meshheading:7912974-Quinoxalines,
pubmed-meshheading:7912974-Retina,
pubmed-meshheading:7912974-gamma-Aminobutyric Acid
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Domoic acid induced release of [3H]GABA in cultured chick retina cells.
|
| pubmed:affiliation |
Department of Fundamental Biology, University of Vigo, Spain.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|