Linked Life Data

791228

Source:http://linkedlifedata.com/resource/pubmed/id/791228

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1976-12-23
pubmed:abstractText
The susceptibility of fertilized sheep ova and oocytes, with and without zonae pellucidae, to lysis by complement plus antibodies in antisera raised against sheep ovaries, oocytes and sperm was examined in vitro. The presence of the zona reduced the proportion of ova lysed (8% v. 57%) and a large proportion of ova with zonae continued development in culture following exposure to antisera. Antisera to ovary and to oocytes lysed a higher proportion of ova than did antisera to sperm (51 and 56% v. 29%). There was no effect of age of fertilized ova (day 2, 4, 6; day 0 = day of oestrus) on susceptibility to lysis. Fluorescein-labelled antibodies from high-titre antisera to ovary and oocytes traversed the zona and attached to blastomeres. Following absorption of the antibody conjugates with sheep serum, fluorescein staining of ova with intact zonae was confined to the zona, whereas with zona-free ova the blastomeres were stained. It appears that the zona may protect embryos from lytic antibodies and it is suggested that the zona may be important in protecting the embryo from immunological attack in vivo.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0004-9417
pubmed:author
pubmed:issnType
Print
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
237-44
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1976
pubmed:articleTitle
Studies on the zona pellucida of sheep ova: immune lysis and localization of antibodies in ova with, and without, zonae.
pubmed:publicationType
Journal Article