| pubmed-article:7911708 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7911708 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7911708 | lifeskim:mentions | umls-concept:C0021756 | lld:lifeskim |
| pubmed-article:7911708 | lifeskim:mentions | umls-concept:C0026724 | lld:lifeskim |
| pubmed-article:7911708 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:7911708 | lifeskim:mentions | umls-concept:C0205039 | lld:lifeskim |
| pubmed-article:7911708 | lifeskim:mentions | umls-concept:C0007592 | lld:lifeskim |
| pubmed-article:7911708 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:7911708 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:7911708 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:7911708 | pubmed:dateCreated | 1994-7-15 | lld:pubmed |
| pubmed-article:7911708 | pubmed:abstractText | Functional analysis of T cells from the bronchial mucosa has been limited by difficulties in extracting T cells from this tissue. Because interleukin-2 (IL-2) is chemotactic for T cells, we determined whether this cytokine could be used to extract T cells from human bronchial wall (BW). Fresh tissue was obtained from 21 patients undergoing surgery for malignancy. Within the BW, 95% of T cells stained for the memory/activation marker CD45RO. When BW sections were incubated with IL-2 for 24 h, 88 to 91% of T cells emigrated into the culture medium. Compared with autologous blood T cells (also exposed to IL-2), these BW T cells expressed CD2 at a greater intensity and showed a fourfold reduction in cloning efficiency in response to phytohemagglutinin, and T-cell clones derived from the BW population displayed a tendency for higher interferon-gamma production. Furthermore, we were also able to extract and clone T cells from bronchoscopic biopsies in four subjects, suggesting that this method will provide a new avenue for examining T-cell function in airway inflammatory diseases. | lld:pubmed |
| pubmed-article:7911708 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7911708 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7911708 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:7911708 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7911708 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:7911708 | pubmed:issn | 1073-449X | lld:pubmed |
| pubmed-article:7911708 | pubmed:author | pubmed-author:HillF SFS | lld:pubmed |
| pubmed-article:7911708 | pubmed:author | pubmed-author:RobinsonB WBW | lld:pubmed |
| pubmed-article:7911708 | pubmed:author | pubmed-author:McMenaminCC | lld:pubmed |
| pubmed-article:7911708 | pubmed:author | pubmed-author:Schon-HegradM... | lld:pubmed |
| pubmed-article:7911708 | pubmed:author | pubmed-author:UphamJ WJW | lld:pubmed |
| pubmed-article:7911708 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7911708 | pubmed:volume | 149 | lld:pubmed |
| pubmed-article:7911708 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7911708 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7911708 | pubmed:pagination | 1608-13 | lld:pubmed |
| pubmed-article:7911708 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:7911708 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7911708 | pubmed:articleTitle | Functional analysis of human bronchial mucosal T cells extracted with interleukin-2. | lld:pubmed |
| pubmed-article:7911708 | pubmed:affiliation | Western Australian Research Institute for Child Health, Subiaco. | lld:pubmed |
| pubmed-article:7911708 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7911708 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:7911708 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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