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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1994-7-15
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pubmed:abstractText |
Functional analysis of T cells from the bronchial mucosa has been limited by difficulties in extracting T cells from this tissue. Because interleukin-2 (IL-2) is chemotactic for T cells, we determined whether this cytokine could be used to extract T cells from human bronchial wall (BW). Fresh tissue was obtained from 21 patients undergoing surgery for malignancy. Within the BW, 95% of T cells stained for the memory/activation marker CD45RO. When BW sections were incubated with IL-2 for 24 h, 88 to 91% of T cells emigrated into the culture medium. Compared with autologous blood T cells (also exposed to IL-2), these BW T cells expressed CD2 at a greater intensity and showed a fourfold reduction in cloning efficiency in response to phytohemagglutinin, and T-cell clones derived from the BW population displayed a tendency for higher interferon-gamma production. Furthermore, we were also able to extract and clone T cells from bronchoscopic biopsies in four subjects, suggesting that this method will provide a new avenue for examining T-cell function in airway inflammatory diseases.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD2,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Phytohemagglutinins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1073-449X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
149
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1608-13
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:7911708-Aged,
pubmed-meshheading:7911708-Antigens, CD2,
pubmed-meshheading:7911708-Antigens, CD3,
pubmed-meshheading:7911708-Antigens, CD45,
pubmed-meshheading:7911708-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:7911708-Antigens, Surface,
pubmed-meshheading:7911708-Asthma,
pubmed-meshheading:7911708-Biopsy,
pubmed-meshheading:7911708-Bronchi,
pubmed-meshheading:7911708-Bronchoscopy,
pubmed-meshheading:7911708-Carcinoma,
pubmed-meshheading:7911708-Cell Separation,
pubmed-meshheading:7911708-Chemotaxis, Leukocyte,
pubmed-meshheading:7911708-Cloning, Molecular,
pubmed-meshheading:7911708-Evaluation Studies as Topic,
pubmed-meshheading:7911708-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:7911708-Humans,
pubmed-meshheading:7911708-Immunoenzyme Techniques,
pubmed-meshheading:7911708-Interferon-gamma,
pubmed-meshheading:7911708-Interleukin-2,
pubmed-meshheading:7911708-Interleukin-4,
pubmed-meshheading:7911708-Lung Neoplasms,
pubmed-meshheading:7911708-Middle Aged,
pubmed-meshheading:7911708-Mucous Membrane,
pubmed-meshheading:7911708-Phytohemagglutinins,
pubmed-meshheading:7911708-Receptors, Immunologic,
pubmed-meshheading:7911708-T-Lymphocytes
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pubmed:year |
1994
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pubmed:articleTitle |
Functional analysis of human bronchial mucosal T cells extracted with interleukin-2.
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pubmed:affiliation |
Western Australian Research Institute for Child Health, Subiaco.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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