Linked Life Data

7911493

Source:http://linkedlifedata.com/resource/pubmed/id/7911493

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-7-13
pubmed:databankReference
pubmed:abstractText
A cDNA encoding a human IL-12R subunit was isolated by expression cloning. This subunit is a 662 amino acid type I transmembrane protein with an extracellular domain of 516 amino acids and a cytoplasmic domain of 91 amino acids. It is a member of the hemopoietin receptor superfamily and is most closely related over its entire length to gp130 and the receptors for granulocyte-CSF (G-CSF) and leukemia-inhibitory factor. When expressed in COS cells, this IL-12R subunit binds both human and murine IL-12 with an apparent affinity of 2 to 5 nM. The transfected COS cells express both monomers and disulfide-linked dimers or oligomers of the IL-12R subunit on their surface. However, unlike the IL-6-induced dimerization of gp130, the oligomerization of the IL-12R subunit is not dependent on binding of IL-12. Only the IL-12R subunit dimers/oligomers but not the monomers bind IL-12 with an affinity of 2 to 5 nM. A polyclonal antiserum raised against this receptor subunit specifically inhibits IL-12-induced proliferation of PHA-activated PBMC. The data are consistent with the hypotheses that 1) a dimer/oligomer of the cloned IL-12R subunit (IL-12R-beta) represents the low affinity IL-12 binding site identified on human lymphoblasts, 2) the cloned receptor subunit is involved in IL-12 signal transduction, and 3) an additional, as of yet unidentified subunit is required to generate a high affinity IL-12R complex.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
153
pubmed:geneSymbol
G-CSF-R, IL-12R, LIF-R
pubmed:owner
NLM
pubmed:authorsComplete
N
pubmed:pagination
128-36
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:7911493-Amino Acid Sequence, pubmed-meshheading:7911493-Antigens, CD, pubmed-meshheading:7911493-Cloning, Molecular, pubmed-meshheading:7911493-Consensus Sequence, pubmed-meshheading:7911493-Cytokine Receptor gp130, pubmed-meshheading:7911493-DNA, Complementary, pubmed-meshheading:7911493-Genes, pubmed-meshheading:7911493-Humans, pubmed-meshheading:7911493-Interleukin-12, pubmed-meshheading:7911493-Interleukins, pubmed-meshheading:7911493-Membrane Glycoproteins, pubmed-meshheading:7911493-Molecular Sequence Data, pubmed-meshheading:7911493-Multigene Family, pubmed-meshheading:7911493-Receptors, Cytokine, pubmed-meshheading:7911493-Receptors, Interleukin, pubmed-meshheading:7911493-Receptors, Interleukin-12, pubmed-meshheading:7911493-Sequence Alignment, pubmed-meshheading:7911493-Sequence Homology, Amino Acid
pubmed:year
1994
pubmed:articleTitle
Expression cloning of a human IL-12 receptor component. A new member of the cytokine receptor superfamily with strong homology to gp130.
pubmed:affiliation
Department of Inflammation/Autoimmune Diseases, Roche Research Center, Hoffmann-La Roche Inc., Nutley, NJ 07110.
pubmed:publicationType
Journal Article, Comparative Study