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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
25
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pubmed:dateCreated |
1994-7-5
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pubmed:abstractText |
The alpha 1-adrenoceptors present in liver membranes from rats, hamsters and mice were characterized using [3H]prazosin. In the liver membranes from the three species a relatively large number of receptors was observed (500-900 fmol/mg of protein) and the affinities for [3H]prazosin were very similar (0.2-0.3 nM). Membrane preincubation with 10 microM chloroethylclonidine markedly decreased [3H]prazosin binding and higher concentrations essentially abolished specific binding of this radioligand. Binding competition experiments indicated the following orders of potency: a) for agonists: oxymetazoline > epinephrine > or = norepinephrine >> methoxamine and b) for antagonists: prazosin > WB 4101 > or = phentolamine = benoxathian > 5-methyl urapidil. The affinity for (+)niguldipine was also low but there was variation between the three species. Total RNA obtained from the liver of these species hybridized with the alpha 1B-adrenergic cDNA probe. The data suggest that these receptors correspond to the alpha 1B subtype.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0024-3205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1995-2003
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:7911220-Adrenergic alpha-Agonists,
pubmed-meshheading:7911220-Adrenergic alpha-Antagonists,
pubmed-meshheading:7911220-Animals,
pubmed-meshheading:7911220-Binding, Competitive,
pubmed-meshheading:7911220-Binding Sites,
pubmed-meshheading:7911220-Cell Membrane,
pubmed-meshheading:7911220-Clonidine,
pubmed-meshheading:7911220-Cricetinae,
pubmed-meshheading:7911220-Dihydropyridines,
pubmed-meshheading:7911220-Liver,
pubmed-meshheading:7911220-Male,
pubmed-meshheading:7911220-Mesocricetus,
pubmed-meshheading:7911220-Mice,
pubmed-meshheading:7911220-Prazosin,
pubmed-meshheading:7911220-Rats,
pubmed-meshheading:7911220-Rats, Wistar,
pubmed-meshheading:7911220-Receptors, Adrenergic, alpha,
pubmed-meshheading:7911220-Species Specificity
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pubmed:year |
1994
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pubmed:articleTitle |
Characterization of the hepatic alpha 1B-adrenoceptors of rats, mice and hamsters.
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pubmed:affiliation |
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México, D. F.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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