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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1994-6-9
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pubmed:abstractText |
The identity of the serotonin (5-HT) receptor(s) that mediate(s) contraction in canine coronary artery and saphenous vein remains controversial. Ring segments of endothelium-denuded coronary artery and helical strips of saphenous vein were suspended in organ chambers for measurement of isometric force. 5-HT, alpha Me-5-HT and sumatriptan contracted both coronary artery and saphenous vein and the non-selective 5-HT receptor antagonist 1-naphthylpiperazine (100nM) blocked 5-HT- and sumatriptan-induced contraction in both tissues. The agonist rank order potency for contraction (5-HT > sumatriptan > alpha Me5-HT > 5-MeOT > 5-MeT) was similar in both tissues and was consistent with that for a 5-HT1D receptor. Oligonucleotide primers specific for the 5-HT1D receptor sequence were designed for use in a polymerase chain reaction (PCR). cDNA derived from total RNA or mRNA from canine tissues was used in the PCR. PCR resulted in the amplification of a 632 base pair sequence in both canine coronary artery and saphenous vein; consistent with that expected for the 5-HT1D receptor. Southern blot analysis, with an oligonucleotide probe internal to the sequence amplified by the PCR primers, confirmed that the sequence amplified by PCR was the 5-HT1D receptor. Thus, the 5-HT1D receptor is expressed in canine coronary artery and saphenous vein and taken together with the pharmacological data, supports the possibility that a 5-HT1D-like receptor mediates contraction in these two tissues.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(1-naphthyl)piperazine,
http://linkedlifedata.com/resource/pubmed/chemical/5-Methoxytryptamine,
http://linkedlifedata.com/resource/pubmed/chemical/5-carboxamidotryptamine,
http://linkedlifedata.com/resource/pubmed/chemical/5-methyltryptamine,
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic alpha-Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Dioxanes,
http://linkedlifedata.com/resource/pubmed/chemical/Idazoxan,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Prazosin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Sumatriptan,
http://linkedlifedata.com/resource/pubmed/chemical/Tryptamines,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-methylserotonin
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pubmed:status |
MEDLINE
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pubmed:issn |
0024-3205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
54
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1671-80
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:7909909-5-Methoxytryptamine,
pubmed-meshheading:7909909-Adrenergic alpha-Antagonists,
pubmed-meshheading:7909909-Animals,
pubmed-meshheading:7909909-Base Sequence,
pubmed-meshheading:7909909-Blotting, Southern,
pubmed-meshheading:7909909-Coronary Vessels,
pubmed-meshheading:7909909-DNA Primers,
pubmed-meshheading:7909909-Dioxanes,
pubmed-meshheading:7909909-Dogs,
pubmed-meshheading:7909909-Dose-Response Relationship, Drug,
pubmed-meshheading:7909909-Gene Expression,
pubmed-meshheading:7909909-Idazoxan,
pubmed-meshheading:7909909-Molecular Sequence Data,
pubmed-meshheading:7909909-Muscle, Smooth, Vascular,
pubmed-meshheading:7909909-Muscle Contraction,
pubmed-meshheading:7909909-Piperazines,
pubmed-meshheading:7909909-Polymerase Chain Reaction,
pubmed-meshheading:7909909-Prazosin,
pubmed-meshheading:7909909-RNA, Messenger,
pubmed-meshheading:7909909-Receptors, Serotonin,
pubmed-meshheading:7909909-Saphenous Vein,
pubmed-meshheading:7909909-Serotonin,
pubmed-meshheading:7909909-Serotonin Receptor Agonists,
pubmed-meshheading:7909909-Sumatriptan,
pubmed-meshheading:7909909-Tryptamines
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pubmed:year |
1994
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pubmed:articleTitle |
Serotonin-induced contraction in canine coronary artery and saphenous vein: role of a 5-HT1D-like receptor.
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pubmed:affiliation |
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285.
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pubmed:publicationType |
Journal Article
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