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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1994-6-6
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pubmed:abstractText |
Construction of a series of chimeric antibodies (murine variable region and human constant region) derived from the murine antibody BIRR1, which recognizes intercellular adhesion molecule 1 (ICAM-1), has revealed differences in the relative binding abilities of the chimeric antibody to antigen. The chimeric antibodies show a ranking of their ability to compete with BIRR1 for antigen on the surface of cells with the order BIRR1 = cIgG1 (100%) > cIgG4 (30%) > cIgG2 (10%) as demonstrated by solid-phase competitive enzyme-linked immunosorbent assay. Papain digestion yielded Fab fragments that were purified to homogeneity. Competitive enzyme-linked immunosorbent assay showed that the chimeric and murine Fab binding constants were equivalent. A solution-phase binding assay (analyzed by size exclusion high performance liquid chromatography) between the intact mAbs and recombinant soluble ICAM-1 further established that the binding constants involving the Fab arms of the two antibodies were equivalent. In summary, the murine and chimeric anti-ICAM-1 antibodies bind cellular ICAM-1 with equivalent affinities but with differing avidities.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
N
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pubmed:pagination |
13048-55
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:7909805-Animals,
pubmed-meshheading:7909805-Antibodies, Monoclonal,
pubmed-meshheading:7909805-Base Sequence,
pubmed-meshheading:7909805-Binding Sites, Antibody,
pubmed-meshheading:7909805-CHO Cells,
pubmed-meshheading:7909805-Cell Adhesion Molecules,
pubmed-meshheading:7909805-Cricetinae,
pubmed-meshheading:7909805-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:7909805-Humans,
pubmed-meshheading:7909805-Immunoglobulin Fab Fragments,
pubmed-meshheading:7909805-Immunoglobulin Isotypes,
pubmed-meshheading:7909805-Intercellular Adhesion Molecule-1,
pubmed-meshheading:7909805-Mice,
pubmed-meshheading:7909805-Molecular Sequence Data,
pubmed-meshheading:7909805-Oligodeoxyribonucleotides,
pubmed-meshheading:7909805-Recombinant Fusion Proteins
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pubmed:year |
1994
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pubmed:articleTitle |
Isotype choice for chimeric antibodies affects binding properties.
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pubmed:affiliation |
Department of Biochemistry, Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, Connecticut 06877-0368.
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pubmed:publicationType |
Journal Article
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